鸭疫里默氏菌感染对鸭肠道菌群结构的影响

doi:10.16431/j.cnki.1671-7236.2021.06.026

Abstract

Abstract: The purpose of this study was to analyze the changes of intestinal microflora in ducks infected with Riemerella anatipestifer (RA) in different periods,and to explore the effect of Riemerella anatipestifer on intestinal microflora and the possible pathogenic mechanism.Using universal primers of 16S V4-V5 region of bacteria,the DNA of duck rectal contents in Riemerella anatipestifer uninfected group (Riemerella anatipestifer infected 0 day group) and Riemerella anatipestifer infected 1,2,3,5,9 and 14 days were amplified.The amplified products were sequenced on Ion S5^TMXL sequencing platform after DNA library construction.The total number of Raw Reads was 4 710 688,with an average of 84 119 sequences per sample.The total number of OTUs annotated to the database was 4 528.Alpha diversity analysis showed that Shannon and Simpson indexes had no significant difference between Riemerella anatipestifer infected groups and uninfected group (P>0.05).The indexes of Chao1,Ace,Observed-species and PD-whole-tree decreased gradually from 0 to 5 d after infection,and increased gradually from 5 to 14 d after infection,especially at 3 and 5 d after infection,which were significantly lower than those in uninfected group (P<0.05).Beta diversity analysis showed that there were significant differences between the uninfected group and the infected groups at 6 time points (P<0.05).The species difference between the uninfected group and 3 d after infection was the largest,followed by 2,5,9,1 and 14 d after infection.The cluster heat map of species abundance showed that the microbial genera with relatively high species abundance were different in the uninfected group and the infected groups at different time points.At the phylum level,Firmicutes,unidentified-Bacteria,Bacteroidetes,Fusobacteria and Proteobacteria were the main pathogens in Riemerella anatipestifer infection groups.At the genus level,Bacteroides was the dominant genus in all infected and uninfected groups.The number of Campylobacter and Fusobacterium in infected groups increased significantly.In this experiment,the intestinal microflora data of Riemerella anatipestifer uninfected group and different infection time groups were obtained.The differences between the infected group and uninfected group were analyzed and compared.Some characteristic bacteria were found,which could provide theoretical basis for the pathogenicity research of Riemerella anatipestifer.

Key words: Riemerella anatipestifer; 16S rDNA; duck; intestinal microflora

CLC Number:

S852.61

TAO Zhiyun, ZHU Chunhong, SHI Zuhao, XU Wenjuan, ZHANG Shuangjie, SONG Weitao, LIU Hongxiang, LI Huifang. Effect of Riemerella anatipestifer Infection on the Microflora of Ducks[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(6): 2129-2139.

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Effect of Riemerella anatipestifer Infection on the Microflora of Ducks

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