北京鸭和连城白鸭胸肌脂质组鉴定与分析

doi:10.16431/j.cnki.1671-7236.2023.03.006

Abstract

Abstract: 【Objective】 The purpose of this experiment was to study the lipid profile of duck breast muscle and investigate the differences between the breast muscle lipidome of Pekin duck and Liancheng White duck.【Method】 28 Pekin ducks and 28 Liancheng White ducks were selected at 6-week-old and their breast muscle tissues were collected to detect the lipid profile used lipidomics techniques based on liquid chromatography-mass spectrometry (LC-MS).The results were analyzed by multivariate statistical analysis,and the differential lipids were screened by fold change,t-test and partial least squares discriminant analysis (PLS-DA).【Result】 A total of 950 lipids were identified in duck breast muscle,covering 6 categories,395 glycerophospholipids (GP),365 glycerolipids (GL),86 sphingolipids (SP),78 fatty acyls (FA),23 sterol lipids (ST) and 3 prenol lipids (PR);The most abundant lipid subgroup was triglycerides (TG) with 283 species.Compared with Pekin duck,18 lipids were screened in Liancheng White duck, 7 were upregulated and 11 were downregulated;Among them,TGs were the most abundant and high in Pekin duck,which were rich in saturated and monounsaturated fatty acids,while these GP containing very long chain polyunsaturated fatty acid (VLC-PUFA) were found to be high in Liancheng White duck.【Conclusion】 In this study,a comprehensive and detailed lipid profile of duck breast muscle was obtained,and 18 differential lipid markers were screened for Pekin duck and Liancheng White duck,this study provided a scientific and theoretical basis for intramuscular lipid metabolism,meat quality research and quality breeding of duck.

Key words: Pekin duck; Liancheng White duck; breast muscle; lipidome; intramuscular lipid

CLC Number:

S834

ZHANG He, LIU Dapeng, TANG Hehe, LIU Tong, YU Daxin, YU Simeng, GAI Kai, HOU Shuisheng, ZHOU Zhengkui. Identification and Analysis of the Lipidome of the Breast Muscle of Pekin Duck and Liancheng White Duck[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 904-912.

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Identification and Analysis of the Lipidome of the Breast Muscle of Pekin Duck and Liancheng White Duck

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