鸭胸肌矿物元素含量全基因组关联分析

doi:10.16431/j.cnki.1671-7236.2023.04.001

Abstract

Abstract: 【Objective】 The aim of this study was to identify candidate genes and molecular markers affecting the contents of mineral elements in breast muscle of ducks by genome-wide association study (GWAS),and analyze the genetic mechanism of mineral element traits.【Method】 The F2 generation resource population of Pekin ducks×Anas platyrhynchos was constructed,and the whole genome was resequenced.The breast muscles of ducks were collected for 8 weeks and the contents of 7 major mineral elements were determined according to the national standard method.Combined with the resequencing data and mineral element phenotypic data,the genes related to element content were identified by whole gene association analysis.【Result】 The phenotypic heritability of the contents of seven mineral elements was P(0.007)＜Fe(0.100)＜Mg(0.120)＜Na(0.140)＜K(0.150)＜Ca(0.190)＜Zn(0.350).The results of correlation analysis showed that the correlation between Mg and K was the highest (r=0.62).There was a high positive correlation between P and Mg as well as K in duck breast,r were 0.43 and 0.56,respectively.A SNP locus significantly related to Zn content were identified by GWAS and reached a significant association (-log₁₀P-value=8.03) at 12 075 283 bp on chromosome 6. 39 SNPs highly correlated with the highest point SNP narrowed the candidate interval to 39 kb,in which there was only one gene SORCS3.Combined with gene functional annotation and transcriptome,SORCS3 was identified as a candidate gene for Zn content variation.The highest point of correlation between SNP and P content at the whole genome level was highly correlated with 260 SNPs(R²＞0.4),all of which were located at chromosome 4 between 17.1-18.6 Mb and contained 27 genes.Combined with correlation analysis,gene functional annotation and transcriptome,six candidate genes were highly related to P content were identified (TUSC3,MICU3,LOC101803915,MTMR7,SLC7A2 and ASAH1 genes).【Conclusion】 The candidate gene SORCS3 of Zn content was identified by GWAS,and combined with transcriptome,six candidate genes related to P content were identified.These results enriched the knowledge system of mineral nutrition of poultry meat,and provided an important theoretical basis for breeding excellent livestock and poultry products.

Key words: duck; breast; mineral elements; GWAS; heritability

CLC Number:

S834

YU Daxin, LIU Wenjing, LIU Dapeng, LIU Tong, ZHANG He, GAI Kai, LI Shunan, ZHANG Beibei, HOU Shuisheng, ZHOU Zhengkui. Genome-wide Association Studies on Mineral Elements in Breast Muscle of Ducks[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1287-1295.

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Genome-wide Association Studies on Mineral Elements in Breast Muscle of Ducks

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