2株海鸭源H3亚型禽流感病毒全基因组分子特征分析

doi:10.16431/j.cnki.1671-7236.2023.04.004

Abstract

Abstract: 【Objective】 This study was aimed to better understand the molecular characteristics of complete genomes of waterfowl infected with H3 subtype Avian influenza virus (AIV) in the coast area of Guangxi.【Method】 Two strains of H3 subtype AIV named A/duck/Guangxi/S11359/2020 (H3N1) and A/duck/Guangxi/S11374/2020 (H3N8) were acquired by one-step RT-PCR amplification of genome segment,then conducted sequencing and evolutionary analysis further in this study.【Result】 The amino acid cleavage sites of HA protein of both H3 subtypes AIV were ³⁴⁰PEKQTR↓GLFG³⁴⁹ each,which was in accordance with the molecular characteristics of low pathogenic AIV,and the 226th and 228th amino acid sites correlated to receptor tropism were Q and T,indicating that were poultry receptor as possible.The BLAST analysis result showed that M and NP genes of H3N1 and PA gene of H3N8 with the highest similarity to H7 subtype AIV was 99.5%,98.7% and 98.9%,respectively,which suggested that genes recombination was occurred between H3 and H7 subtypes AIV.The genes of both H3 subtype AIV shared the highest similarity with that of waterfowl-origin H3,H4,H6,H7 and H9 subtypes AIV from Vietnam,Bangladesh and South Korea countries around China,which indicated that the isolates possessed the same evolutionary source.Genetic analysis indicated that all genes of both H3 subtype AIV isolates belonged to the Eurasian branch,the genetic distance of those was the closest to waterfowl-origin influenza virus strains,closer to canine and feline-origin strains,close to human and porcine-origin strains,but in addition to NS gene of H3N1 isolate which got the closest genetic distance to A/Aichi/2/1968 derived from Japanese earlier,suggesting that genes exchanged could occurred between them.Recombination sign of HA gene of H3N8 isolate was detected,the recombinant parent strains of that were A/duck/Beijing/40/04 and A/canine/Beijing/20121215-34/2012 strains,with the similarity was 93.1% and 97.1%,respectively.【Conclusion】 The source and genetic characteristics of genes of both sea duck-origin H3 subtype AIV from Guangxi was complicated and divergent.

Key words: sea duck; H3 subtype Avian influenza virus; genetic evolution

CLC Number:

S852.65⁺7

XIE Shouyu, WANG Ruimin, CUI Pengfei, SHI Kaichuang, WEI Xiankai, FENG Shuping, LONG Feng, QU Sujie, LU Wenjun, HUANG Jinshan, HUANG Fengmei, WEN Xinrui, YIN Yanwen, DENG Guohua. Molecular Characteristics of Complete Genomes of Two H3 Subtype Avian Influenza Virus Isolates Derived from Sea Duck in Guangxi Province[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1319-1328.

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Molecular Characteristics of Complete Genomes of Two H3 Subtype Avian Influenza Virus Isolates Derived from Sea Duck in Guangxi Province

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