不同精粗比饲粮对绵羊瘤胃体外发酵和共轭亚油酸产量的影响

doi:10.16431/j.cnki.1671-7236.2022.05.012

Abstract

Abstract: 【Objective】 On the basis of adding 2% soybean oil,this experiment was conducted to investigate the effects of concentrate to forage ratio on ruminal fermentation and conjugated linoleic acid production of sheep in vitro.【Method】 Rumen fluid from sheep was used as the inoculum,and 7 levels of dietary concentrate to forage ratio (concentrate to forage ratio was 2∶8,3∶7,4∶6,5∶5,6∶4,7∶3,8∶2 respectively) were used as the substrate,2% soybean oil was added into glass fermentation tubes during the in vitro 48 h fermentation.The trail was repeated twice with four parallels in each group.【Result】 ①The concentration of acetic acid in level 4∶6 was the highest and significantly higher than those with others (P<0.01).The concentration of valeric acid increased firstly and then decreased with the increase of concentrate to forage ratio,and the concentration was hightest in level 4∶6.The concentration of total volatile acid in level 4∶6 was significantly higher than that in levels 2∶8,3∶7,6∶4 and 7∶3 (P<0.05).There was no significant difference in the concentration of propionic acid,butyric acid,isobutyric acid,isovaleric acid and the ratio of acetic acid to propionic acid between other treatment levels (P>0.05).pH value was highest at level 8∶2.②The dry matter degradation rate of level 4∶6 was significantly higher than levels 3∶7 and 5∶5 (P<0.05).The production of methane in level 4∶6 was the lowest and significantly lower than that in level 6∶4 (P<0.01).Gas production of level 6∶4 was higher than levels 2∶8,3∶7 and 5∶5 (P<0.05),but there was no significant difference with level 4∶6 (P>0.05).③The concentration of ammonia nitrogen increased first and then decreased,which the highest point located in level 4∶6,extremely significantly higher than that in levels 2∶8 and 8∶2 (P<0.01),significantly higher than that in levels 3∶7 and 6∶4 (P<0.05).The concentrations of microbial protein and acetic acid in level 4∶6 were the highest and significantly higher than those with others (P<0.01).④There was no significant difference in the concentration of conjugated linoleic acid in each group (P>0.05).【Conclusion】 On the basis of supplemented 2% soybean oil,the level of 4∶6 of dietary concentrate to forage ratio was the best value for ruminal fermentation and dry matter degradation of sheep in vitro.

Key words: concentrate to forage ratio; soybean oil; in vitro fermentation; conjugated linoleic acid

CLC Number:

S816.79

TIAN Xiya, SUN Yang, NIU Huijing, YU Tianli, HE Zhenlian, XIA Chengqiang, PEI Caixia. Effects of Dietary with Different Concentrate to Forage Ratio on Ruminal Fermentation and Conjugated Linoleic Acid Production of Sheep in vitro[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1707-1714.

References

[1] 占今舜,霍俊宏,胡耀,等.不同精粗比全混合日粮对努比亚山羊肉品质、血清指标和器官发育的影响[J].草业学报,2020,29(10):139-148. ZHAN J S,HUO J H,HU Y,et al.Effects of total mixed rations with different concentrate:Roughage ratios on meat quality,serum indexes and organ development of nubian goats[J].Acta Prataculturae Sinica,2020,29(10):139-148.(in Chinese)
[2] OLIJHOEK D W,LOVENDAHL P,LASSEN J,et al.Methane production,rumen fermentation,and diet digestibility of holstein and jersey dairy cows being divergent in residual feed intake and fed at 2 forage-to-concentrate ratios[J].Journal of Dairy Science,2018,101(11):9926-9940.
[3] LILLIS L,BOOTS B,KENNY D A,et al.The effect of dietary concentrate and soya oil inclusion on microbial diversity in the rumen of cattle[J].Journal of Applied Microbiology,2011,111(6):1426-1435.
[4] VARGAS J E,ANDRES S,LOPEZ-FERRERAS L,et al.Dietary supplemental plant oils reduce methanogenesis from anaerobic microbial fermentation in the rumen[J].Scientific Reports,2020,10(1):1613.
[5] CANDYRINE S C,JAHROMI M F,EBRAHIMI M,et al.Oil supplementation improved growth and diet digestibility in goats and sheep fed fattening diet[J].Asian-Australasian Journal of Animal Sciences,2019,32(4):533-540.
[6] 魏宏阳,王加启.添加游离不饱和脂肪酸或植物油对瘤胃微生物体外发酵及微晶纤维素降解的影响[J].畜牧兽医学报,2004,5:491-497. WEI H Y,WANG J Q.Effects of free unsaturated fatty acids and vegetable oil on pure cellulose degradation and fermentation by mixed ruminal microbes in vitro[J].Chinese Journal of Animal and Veterinary Sciences,2004,5:491-497.(in Chinese)
[7] HENNESSY A A,ROSS P R,FITZGERALD G F,et al.Sources and bioactive properties of conjugated dietary fatty acids[J].Lipids,2016,51(4):377-397.
[8] NUDDA A,CANNAS A,COREEDU F,et al.Sheep and goats respond differently to feeding strategies directed to improve the fatty acid profile of milk fat[J].Animals,2020,10(8):1290.
[9] 于伽,周薇,陈娜,等.不同精粗比日粮添加α-亚麻酸对瘤胃体外发酵参数及生物氢化代谢产物的影响[J].中国饲料,2016,20:17-22. YU J,ZHOU W,CHEN N,et al.Effects of α-linolenic acid added to dietary with different proportion of concentration and roughage on the ruminal fermentation characteristics and the bio-hydrogenation[J].China Feed,2016,20:17-22.(in Chinese)
[10] 马秀花,周玉香.羊肉共轭亚油酸调控技术研究进展[J].动物营养学报,2020,32(8):3568-3576. MA X H,ZHOU Y X.Research progress on regulation technology of conjugated linoleic acid in mutton[J].Chinese Journal of Animal Nutrition,2020,32(8):3568-3576.(in Chinese)
[11] CASTRO T,MARTINEZ D,ISABLE B,et al.Vegetable oils rich in polyunsaturated fatty acids supplementation of dairy cows' diets:Effects on productive and reproductive performance[J].Animals (Basel),2019,9(5):205.
[12] ZHAO J,MA X,JIN Y,et al.Energy requirements for the maintenance and growth of dorper-jinzhong crossbred ram lambs[J].Italian Journal of Animal Science,2016,15(1):94-102.
[13] MENKE K H,STEINGASS H.Estimation of the energetic feed valueobtained from chemical analysis and in vitro gas production using rumen fluid[J].Animal Research and Development,1988,28:7-55.
[14] MAKKAR H P,SHARMA O P,DAWRA R K,et al.Simple determination of microbial protein in rumen liquor[J].Journal of Dairy Science,1982,65(11):2170-2173.
[15] BRODERICK G A,KANG J H.Automated simul taneous determination of ammonia and total amino acids in ruminal fluid and in vitro medial[J].Journal of Dairy Science,1980,63(1):64.
[16] RATTRAY P V,GARRETT W N,EAST N E,et al.Growth,development and composition of the ovine conceptus and mammary gland during pregnancy[J].Journal of Animal Science,1974,38(3):613-626.
[17] 卜登攀,刘仕军,周凌云,等.牛奶脂肪酸及CLA的分析方法的改进[J].中国农学通报,2006,7:1-4. BU D P,LIU S J,ZHOU L Y,et al.Low toxic methods for determining milk fatty acid composition and CLA isomers[J].China Agricultural Science Bulletin,2006,7:1-4.(in Chinese)
[18] BAYAT A R,VENTTO L,KAIRENIUS P,et al.Dietary forage to concentrate ratio and sunflower oil supplement alter rumen fermentation,ruminal methane emissions,and nutrient utilization in lactating cows[J].Translational Animal Science,2017,3:277-286.
[19] 王志敬,吴征敏,庄桂锋,等.不同精粗比日粮对雷州山羊体外瘤胃发酵的影响[J].中国畜牧兽医,2017,44(8):2303-2310. WANG Z J,WU Z M,ZHUANG G F,et al.Effects of diets with different concentrate to forage ratios on in vitro rumen fermentation of Leizhou goats[J].China Animal Husbandry & Veterinary Medicine,2017,44 (8):2303-2310.(in Chinese)
[20] 王正文,魏玉明,杨发荣,等.不同日粮精粗比下藜麦秸秆与高粱秸秆的组合效应[J].草业科学,2021,38(8):1615-1625. WANG W W,WEI Y M,YANG F R,et al.Study on the associative effects of quinoa andsorghum straw under different diet ratios of concentrate to roughage[J].Pratacultural Science,2021,38 (8):1615-1625.(in Chinese)
[21] YANEZ-RUIZ D R.Design,implementation and interpretation of in vitro batch culture experiments to assess enteric methane mitigation in ruminants-A review[J].Animal Feed Science and Technology,2016,216:1-18.
[22] LETTAT A,BENCHAAR C.Diet-induced alterations in total and metabolically active microbes within the rumen of dairy cows[J].PLoS One,2013,8(4):e60978.
[23] QI X L,WANG J,YUE H Y,et al.Trans10,cis12-conjugated linoleic acid exhibits a stronger antioxidant capacity than cis9,trans11-conjugated linoleic acid in primary cultures of laying hen hepatocytes[J].Journal of Poultry Science,2018,97(12):4415-4424.
[24] 黎晓磊,徐其红.蛋白质来源与粗精料比例对瘤胃体外发酵及微生物的影响[J].中国饲料,2020,22:69-72. LI X L,XU Q H.Effects of protein source and rough concentrate feed ratio on rumen in vitro fermentation and microorganism[J].China Feed,2020,22:69-72.(in Chinese)
[25] 谭健,王荣,张秀敏,等.体外法研究亚麻籽油对瘤胃甲烷、氢气产量和脂肪酸组成的影响[J].动物营养学报,2021,33(11):6492-6500. TAN J,WANG R,ZHANG X M,et al.Effects of linseed oil on rumen methane,hydrogen production and fatty acid composition using in vitro method[J].Chinese Journal of Animal Nutrition,2021:33(11):6492-6500.(in Chinese)
[26] 王啸林,冯冠智,张城瑞,等.乳酸菌和纤维素酶马铃薯渣与玉米秸秆混合青贮发酵品质、有氧稳定性和体外发酵参数的影响[J].中国畜牧杂志,2022,58(4):201-207. WANG X L,FENG G Z,ZHANG C R,et al.Effects of lactic acid bacteria and cellulase on fermentation quality,aerobic stability and in vitro fermentation parameters of potato residue and corn straw mixed silage with different proportion[J].Chinese Journal of Animal Science,2022,58(4):201-207.(in Chinese)
[27] 曾钰,谢昕廷,彭忠利,等.不同精粗比饲粮中添加酵母培养物对牦牛瘤胃体外发酵参数的影响[J].动物营养学报,2019,31(12):5582-5594. ZENG Y,XIE X T,PENG Z L,et al.Effects of yeast culture supplementation in diet with different concentrate to forage ratios on in vitro fermentation parameters of yaks[J].Chinese Journal of Animal Nutrition,2019,31(12):5582-5594.(in Chinese)
[28] PHESATCHA K,PHESATCHA B,WANAPAT M,et al.Roughage to concentrate ratio and saccharomyces cerevisiae inclusion could modulate feed digestion and in vitro ruminal fermentation[J].Journal of Veterinary Science,2020,7(4):151.
[29] MARIZ L D,AMARAL P M,VALADARES F S,et al.Dietary protein reduction on microbial protein,amino acids digestibility,and body retention in beef cattle.Ⅰ.Digestibility sites and ruminal synthesis estimated by purine bases and 15N as markers[J].Journal of Animal Science,2018,96(6):2463-2464.
[30] FUENTES M C,CALSAMIGLIA S,CARDOZO P W,et al.Effect of pH and level of concentrate in the diet on the production of biohydrogenation intermediates in a dual-flow continuous culture[J].Journal of Dairy Science,2009,92(9):4456-4466.

Effects of Dietary with Different Concentrate to Forage Ratio on Ruminal Fermentation and Conjugated Linoleic Acid Production of Sheep in vitro

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	GUO Yanxia, LI Mengwei, PENG Lijuan, PENG Kaiping, TANG Zhenhua, LIANG Xin, XIE Fang, YANG Chengjian. Effects of Sodium Nitrate on Methane Production and Fatty Acid Hydrogenation Process of Buffalo in vitro Fermentation [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(7): 2071-2080.
[2]	YU Wenjing, WANG Chao, YI Xianfeng, LIN Bo, PANG Tiande, HUANG Zhichao. Evaluation of Nutritional Value of Various Common Ruminant Feeds in Subtropical by in vitro Fermentation [J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(12): 3530-3537.
[3]	LIU Huanhuan, GUO Yanhua, ZHANG Qiaoe, FENG Yuan, LIANG Xiaojun. Effects of Different Conjugated Linoleic Acid Levels on Serum Biochemical Parameters and Muscle Fatty Acids in Eliminated Angus Cows [J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(12): 3581-3589.
[4]	ZHENG Weicai, HAO Xiaoyan, ZHANG Chunxiang, XIANG Binwei, ZHANG Wenjia, WEN Haoyu, ZHANG Jianxin. Effects of Saccharomyces cerevisiae and Bacillus licheniformis on Rumen Fermentation in Sheep in vitro [J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(11): 3208-3215.
[5]	YANG Mei, MA Jie, YANG Tai, YAN Zhaoming, CHEN Qinghua. Biological Function of Conjugated Linoleic Acid and Its Application in Animal Production [J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(11): 3216-3224.
[6]	LI Yan, DING Gengzhi, YAO Qianqian, WU Hao, MENG Qingxiang, ZHOU Zhenming. Effect of Saccharomyces cerevisiae on Plasma Metabolomics of Steers Fed Diets with Different Concentrate to Forage Ratios [J]. , 2019, 46(1): 89-100.
[7]	XU Xiang-ting, WANG Bao-liang, CHENG Guang-min, ZHANG Yong-cui, LIU Hong-bo. Effect of Different Dietary Concentrate to Forage Ratios on Growth Performance, Serum Biochemical Indexes and Economic Benefits of Dorper Sheep [J]. , 2016, 43(3): 668-675.
[8]	ZENG Yan-xia, CHEN Zhi-long, WANG Lin, ZHAO Chen, ZHENG Chen. Effects of Adding Mannan Oligosaccharides to Diets with Different Concentrate to Forage Ratios on Ruminal Fermentation of Sheep in vitro [J]. , 2016, 43(1): 68-75.
[9]	ZHAO Chen, CHEN Zhi-long, ZENG Yan-xia, WANG Lin, ZHENG Chen. Effect of Adding Different Mannan Oligosaccharide to Diets with Different Concentrate to Forage Ratios on Rumen Degradability of Fiber in Sheep [J]. , 2015, 42(12): 3210-3216.
[10]	LI Qing, JIN Wei-xing, LI Xiao, WANG Yan, ZHANG Hui-ling. Effects of Branched Chain Amino Acids on Sheep Rumen Microbial Fermentation in vitro Based on Different Concentrate to Forage Ratios [J]. , 2014, 41(9): 81-88.
[11]	ZHAO Yun-jun, MENG Qing-xiang, ZHANG Jun-yu, GAO Li, YAO Fang. Effect of Dietary Concentrate to Forage Ratio on Rumen Fermentation and Microbial Synthesis of Niacin,Niacinamide in vitro [J]. , 2014, 41(11): 101-106.
[12]	ZHAO Xiao-jing, CAO Yu-feng. Research Progress on Manipulation about Beef Abundance of Functional Fat Acid Production Technology [J]. , 2012, 39(10): 117-121.
[13]	WANG Zhi-you;HOU Sheng-zhen;FAN Yong-tao. Effect of Different Dietary Concentrate to Forage Ratio on Gain of Tibetan Lamb [J]. , 2011, 38(7): 233-235.
[14]	HUO Yin-qiang;TANG Shang-wen. The Biological Effects of Conjugated Linoleic Acid and its Use as Supplements in Pig Diets [J]. , 2011, 38(10): 30-33.
[15]	LIU Ze-xue;XIE Liang-rong;FU Lin-sheng;SHEN Feng. Compare Research of Soybean Oil and Oil Powder on Performance of Weaned Piglets [J]. , 2010, 37(9): 17-19.