酿酒酵母和地衣芽孢杆菌对绵羊瘤胃体外发酵的影响

doi:10.16431/j.cnki.1671-7236.2019.11.009

Abstract

Abstract: The aim of this experiment was to study the effect of Saccharomyces cerevisiae and Bacillus licheniformis on rumen fermentation of sheep by in vitro culture method.Six fermentation substrates were prepared by adding 6×10¹¹ CFU/kg Saccharomyces cerevisiae and 5 levels of Bacillus licheniformis (0 (C1 group)、1×10¹¹(C2 group)、2×10¹¹(C3 group)、3×10¹¹(C4 group) and 4×10¹¹ CFU/kg (C5 group))were added to the diet with a concentrate to forage ratios of 50:50,and the control group was set with no Saccharomyces cerevisiae and Bacillus licheniformis (C group).The gas production parameters,CH₄ concentration,ruminal fermentation parameters and nutrient degradation rates were determined after fermented for 48 h.The result showed as follows:①The combined effects of Saccharomyces cerevisiae and Bacillus licheniformis had significant effects on the cumulative gas production at 24 and 48 h,the gas production of fast fermentation,the gas production of slow fermentation,the potential gas production and the gas production rate (P<0.05),and they reached the maximum at the addition of 2×10¹¹CFU/kg Bacillus licheniformis.②The interaction of Saccharomyces cerevisiae and Bacillus licheniformis had no significant effect on pH value (P>0.05),but they could significantly affect ammoniacal nitrogen (NH₃-N) and microbial proteins (MCP) yields (P<0.05).The concentration of NH₃-N reached the minimum and MCP reached the maximum when the dosage of Bacillus licheniformis was 2×10¹¹ CFU/kg.③The interaction effect of Saccharomyces cerevisiae and Bacillus lichenformis on the dry matter degradation rate (DMD),organic matter degradation rate (OMD) and metabolic energy (ME) were significant (P<0.05),but had no significant effects on neutral detergent fiber degradation rate (NDFD) and acid detergent fiber degradation rate (ADFD) (P>0.05).The DMD,OMD,NDFD and ME reached the maximum while ADFD reached the minimum at the addition of 2×10¹¹CFU/kg Bacillus lichenformis.In conclusion,the Saccharomyces cerevisiae and Bacillus lichenformis supplement had significant interaction effects at 6×10¹¹ and 2×10¹¹ CFU/kg on rumen fermentation of sheep in vitro.

Key words: Saccharomyces cerevisiae; Bacillus lichenformis; sheep; in vitro fermentation

CLC Number:

S816

ZHENG Weicai, HAO Xiaoyan, ZHANG Chunxiang, XIANG Binwei, ZHANG Wenjia, WEN Haoyu, ZHANG Jianxin. Effects of Saccharomyces cerevisiae and Bacillus licheniformis on Rumen Fermentation in Sheep in vitro[J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(11): 3208-3215.

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Effects of Saccharomyces cerevisiae and Bacillus licheniformis on Rumen Fermentation in Sheep in vitro

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