不同种类脂肪酸对延边黄牛骨骼肌卫星细胞成脂转分化的影响

doi:10.16431/j.cnki.1671-7236.2020.04.003

Abstract

Abstract: The purpose of this study was to investigate the effects of different kinds of fatty acid monomers (oleic acid,stearic acid,palmitoleic acid and palmitic acid) on the skeletal muscle satellite cells (BSC) of Yanbian Yellow cattle,and analyze the expression of genes involved in lipogenesis and the effects on the formation of lipid droplets.Skeletal muscle satellite cells were isolated from 18-month-old Yanbian Yellow cattle semi-membrane muscle for in vitro culture,and 100 mmol/L oleic acid,stearic acid,palmitoleic acid and palmitic acid were added to the differentiation medium for 96 h,Real-time quantitative PCR was used to detect the expression of peroxisome proliferator-activated receptor gamma (PPARγ),sterol-regulated binding protein 1 (SREBP1),stearyl coenzyme A desaturase (SCD) and CCAAT/enhancer binding protein alpha (C/EBPα).Oil red O staining results showed that lipid droplets were formed in all fatty acid treatments compared with control cells.Oleic acid and palmitoleic acid treatments had more lipid droplets and larger lipid droplets than palmitic acid and stearic acid treatments.Real-time quantitative PCR results showed that the addition of unsaturated fatty acids such as oleic acid and palmitoleic acid to the skeletal muscle satellite cells of Yanbian Yellow cattle increased the expression of PPARγ,SREBP1 and C/EBPα genes,and inhibited the expression of SCD gene.The addition of saturated fatty acids (stearic acid and palmitic acid) also significantly increased the expression of SCD gene while promoting the expression of PPARγ,SREBP1 and C/EBPα genes(P < 0.05).The results indicated that the addition of fatty acids could induce the transdifferentiation of the skeletal muscle satellite cells to adipocytes in Yanbian Yellow cattle.

Key words: Yanbian Yellow cattle; skeletal muscle satellite cells; fatty acids; lipid droplets; oil red O staining

CLC Number:

S963.73⁺3

ZHANG Junfang, YAN Yan, CUI Yan, SUN Bin, WANG Ying, SUN Jianfu, JIN Xin, YAN Changguo, LI Xiangzi. Effects of Different Fatty Acids on Adipogenic and Transdifferentiation of Skeletal Muscle Satellite Cells in Yanbian Yellow Cattle[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(4): 992-999.

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Effects of Different Fatty Acids on Adipogenic and Transdifferentiation of Skeletal Muscle Satellite Cells in Yanbian Yellow Cattle

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