MSTN基因对牛肌动蛋白细胞骨架调节通路的影响

doi:10.16431/j.cnki.1671-7236.2021.10.006

Abstract

Abstract: In order to explore the mechanism of myostatin (MSTN) on the growth and development of bovine skeletal muscle, quantitative proteomics and phosphorylated proteomics were used to analyze the differences of protein and phosphorylated modification levels in leg gluteal muscle of wild type Mongolian cattle (MG. WT) and MSTN^+/- Mongolian cattle (MG. MSTN^+/-). The established model of muscle differentiation of bovine skeletal muscle satellite cells in vitro was used to detect the interference effect of the designed and synthesized MSTN siRNA (si-MSTN). At the same time, Real-time quantitative PCR and Western blotting were used to detect the expression of mRNA and protein levels of genes related to actin cytoskeleton regulatory pathway in proliferative (GM) and day 3 of differentiation (DM3) bovine skeletal muscle satellite cells transfected with si-MSTN, and study the effect of knockout MSTN expression on actin cytoskeleton regulatory pathway. The results showed that 16 genes related to actin cytoskeleton regulatory pathway were up-regulated in muscle tissue of MSTN^+/- Mongolian cattle, the expression level of MSTN in si-MSTN transfected cells was extremely significantly decreased (P<0.01). In si-MSTN transfected GM phase bovine skeletal muscle satellite cells, the mRNA levels of ENAH, ACTN4 and Cdc42 genes related to actin cytoskeleton regulatory pathway were significantly increased (P<0.05), and the protein levels of PFN1, RhoA and ACTN4 were significantly or extremely significantly increased (P<0.05;P<0.01). In si-MSTN transfected DM3 bovine skeletal muscle satellite cells, the mRNA levels of ENAH, CFL1, SCIN and Cdc42 genes were significantly increased (P<0.05), the mRNA level of RhoA gene was extremely significantly increased (P<0.01), and the protein levels of PFN1 and ACTN4 were significantly increased (P<0.05). The results of this study indicated that interfering with MSTN could promote the expression of genes related to actin cytoskeleton regulatory pathway, and explore the molecular mechanism that MSTN might mediate actin cytoskeleton regulatory pathway to affect the proliferation and myogenic differentiation of bovine skeletal muscle satellite cells, which provided reference for further study of the regulatory mechanism of MSTN on bovine myogenic differentiation.

Key words: bovine; MSTN; skeletal muscle satellite cells; actin cytoskeleton regulatory pathway; proliferation; myogenic differentiation

CLC Number:

SHENG Hui, GUO Yiwen, ZHANG Linlin, TAN Haoyun, HU Debao, LI Xin, DING Xiangbin, GUO Hong. Effect of MSTN Gene on Actin Cytoskeleton Regulatory Pathway[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(10): 3554-3564.

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Effect of MSTN Gene on Actin Cytoskeleton Regulatory Pathway

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