高通量测序分析5例进口鱼粉鱼源性成分构成

doi:10.16431/j.cnki.1671-7236.2021.01.010

Abstract

Abstract: In order to reveal the composition of fishmeal and ensure the quality of fishmeal,high-throughput sequencing technology was used to sequence 5 imported fishmeal samples from different countries,and the composition of fishmeal derived from fish was analyzed based on COI gene and its abundance.After DNA was extracted from fishmeal samples,fish COI gene amplification primers were used for PCR amplification to obtain fish mixed COI gene sequence products.The amplified products were sent to the sequencing company for high-throughput sequencing.The COI gene sequencing data were compared with the fish related databases,and the species-derived components of fishmeal were analyzed through the comparison results.The results showed that 5 fishmeal samples were successfully obtained,and the main component of white fishmeal was cod.The composition of red fishmeal was diverse,including herring,swordfish and so on.The data were statistically analyzed by Python,and the COI gene abundance map of fish after PCR amplification was drawn to further intuitively indicate the composition of raw materials in fishmeal samples.The results showed that high-throughput sequencing technology could be used in the quality evaluation of fishmeal,which was helpful to quickly understand the composition of fishmeal and guide its quality and use.At the same time,it was suggested that this technology could be further applied to the monitoring of fishmeal adulteration and pathogenic microorganisms,which was of great significance to ensure the quality of fishmeal products and maintain the normal market order.

Key words: high throughput sequencing; fishmeal; component analysis; COI gene abundance

CLC Number:

S816

YUAN Xiangfen, WU Shaoqiang, LIN Xiangmei, LYU Jizhou. Analysis of Fish-derived Components of Five Imported Fishmeal Samples via High Throughput Sequencing Method[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(1): 93-100.

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Analysis of Fish-derived Components of Five Imported Fishmeal Samples via High Throughput Sequencing Method

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