无铜饲料对小鼠肠道菌群的影响

doi:10.16431/j.cnki.1671-7236.2017.10.009

Abstract

Abstract:

Copper is an essencial metal for animals to maintain fat metabolism and other important reactions.In order to study the effect of copper deficient diets on composition of intestinal microbiota and their metabolic pathways, 20 health C57BL/6 adult mice with similar body weight were chosen and fed chow diet for 7 d,and then divided into 2 groups.The mice in control group fed with copper deficient diet and 25 mg/L copper water while the experimental group mice were fed copper deficient diet and copper free water for one month.At the end of the test,the blood samples were collected to determine the ceruloplasmin content,and the caecum content were used to analyze the 16S rRNA genome by high throughput sequencing. The results showed that compared with control group,the ceruloplasmin content in experimental group was extremely significantly decreased (P < 0.001).And there were 5 siginificant differences microorganism in phylum level and 43 in genus level (P<0.05). There were 19 significant different pathways in the second grade of KEGG (P<0.05),in which metabolic-related functions were lipid metabolism, glycine biosynthesis and metabolism,metabolism of terpenoids and polyketides,biosynthesis of other secondary metabolisms and amino acid metabolism,indicating that the absence of copper in diets could alter the structure of some microbial flora and the changes of the relevant functions. It provided an important idea for studying the function of copper in the structure and metabolism of intestinal colonies in mice.

Key words: copper; gut microbial colonies; high throughput sequencing; metabolism

CLC Number:

S816.72

WANG Ya-jun, TAO Cong, LI Kui, WANG Yan-fang. Effect of Copper Deficient Diet on Gut Microbiota in Mice[J]. , 2017, 44(10): 2886-2896.

References

[1] Knop M,Dang T Q,Jeschke G. Copper is a cofactor of the formylglycine generating enzyme[J]. Chembiochem, 2017,18(2):161-165.
[2] Kanthlal K,Joseph J,Baskaran-Pillai A K,et al.Neural effects in copper deficient Menkes disease:ATP7A-a distinctive marker[J]. Asian Pacific Journal of Tropical Disease,2016,6(8):668-672.
[3] Medici V, Shibata N M, Kharbanda K K,et al. Wilson's disease:Changes in methionine metabolism and inflammation affect global DNA methylation in early liver disease[J].Hepatology,2013,57(2):555.
[4] 冯丽,文茂瑶,王万琴,等. 126例Wilson's病患者临床特点分析[J]. 四川大学学报(医学版),2016,47(1):128-130.
[5] 叶雷,闫亚丽陈庆森,等. 高通量测序技术在肠道微生物宏基因组学研究中的应用[J].中国食品学报,2016,16(7):216-223.
[6] Krishnamoorthy L,Cotruvo J A Jr,Chan J,et al. Copper regulates cyclic-AMP-dependent lipolysis[J]. Nature Chemical Biology,2016,12(8):586.
[7] 郭彤,许梓荣. 铜离子对引起仔猪腹泻的大肠杆菌K88杀菌机理的研究[J].中国预防兽医学报,2004,26(2):127-130.
[8] 冷向军,王康宁.高铜对早期断奶仔猪消化酶活性、营养物质消化率和肠道微生物的影响[J].饲料研究,2001,4:28-29.
[9] 邹君彪,贺建华.高铜对仔猪肠道微生物作用机制的研究[J].饲料研究,2007,3:45-46.
[10] Lozupone C A, Stombaugh J I,Gordon J I,et al. Diversity,stability and resilience of the human gut microbiota[J]. Nature,2012,489(7415):220-230.
[11] Moschen A R,Kaser S,Tilg H. Non-alcoholic steatohepatitis:A microbiota-driven disease[J]. Trends in Endocrinology & Metabolism,2013,24(11):537-545.
[12] 皮宇,高侃,朱伟云.动物宿主-肠道微生物代谢轴研究进展[J]. 微生物学报,2017,57(2):161-169.
[13] 方南元,孙晓琦,薛博瑜,等. 肠道微生物在非酒精性脂肪肝发病中的研究进展[J]. 中国老年学杂志,2016,36(2):469-471.
[14] Sonnenburg J L,Xu J,Leip D D, et al. Glycan foraging in vivo by an intestine-adapted bacterial symbiont[J]. Science,2005, 307(5717):1955-1959.
[15] Turnbaugh P J,Bäckhed F,Fulton L,et al. Diet-induced obesity is linked to marked but reversible alterations in the mouse distal gut microbiome[J]. Cell Host & Microbe,2008,3(4):213-223.
[16] Edgar R C. Search and clustering orders of magnitude faster than BLAST[J]. Bioinformatics,2010,26(19):2460-2461.
[17] 许晴,张放,张中旗,等.Simpson指数和Shannon-Wiener指数若干特征的分析及"稀释效应"[J].草业科学,2011,28(4):527-531.
[18] Ramette A. Multivariate analyses in microbial ecology[J]. Fems Microbiology Ecology,2007,62(2):142-160.
[19] White J R,Nagarajan N,Pop M. Statistical methods for detecting differentially abundant features in clinical metagenomic samples[J]. PLoS Computational Biology,2009,5(4):e1000352.
[20] Kemp P F,Aller J Y. Bacterial diversity in aquatic and other environments:What 16S rDNA libraries can tell us[J].Fems Microbiology Ecology,2004,47(47):161-177.
[21] 侯振江,孟晨阳. 铜代谢及其临床意义[J]. 微量元素与健康研究,2002,19(3):68-70.
[22] 武娜,朱宝利.人体肠道微生物与健康[J].科学,2013,65(2):13-16.
[23] Handelsman J,Rondon M R, Brady S F,et al. Molecular biological access to the chemistry of unknown soil microbes:A new frontier for natural products[J]. Cell Chemical Biology,1998,5(10):245-249.
[24] 董桂清,钱立群,金志娟. 肝豆状核变性血清铜和铜蓝蛋白测定的意义[J].宁夏医学杂志,2003,25(2):115.
[25] 姬玉娇,祝倩,耿梅梅,等.高、低营养水平饲粮对环江香猪结肠菌群结构及代谢物的影响[J].微生物学报,2015,7:1650-1659.
[26] Woting A,Blaut M.The intestinal microbiota in metabolic disease[J]. Nutrients,2016,8(4):202-221.
[27] Ley R E,Bäckhed F,Turnbaugh P,et al. Obesity alters gut microbial ecology[J].Proc Natl Acad Sci USA,2005,102(31):11070-11075.
[28] Turnbaugh P J,Ley R E,Mahowald M A,et al. An obesity-associated gut microbiome with increased capacity for energy harvest[J]. Nature,2006,444(7122):1027-1031.
[29] Smith E A,Macfarlane G T.Dissimilatory amino acid metabolism in human colonic bacteria[J].Anaerobe,1997,3(5):327-337.

Effect of Copper Deficient Diet on Gut Microbiota in Mice

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