动物益生菌短短芽胞杆菌FJAT-1501-BPA制剂的制备及其益生特性评价

doi:10.16431/j.cnki.1671-7236.2017.10.010

Abstract

Abstract:

The composition of colloidal suspension agent of Brevibacillus brevis FJAT-1501-BPA was optimized to obtain the stable long-term storage agent,at the same time,the evaluation of this strain as a probiotic strain was conducted in this paper. The effects of different stabilizers,pH,NaCl and preservative concentrations on the preservation characteristics of this strain were studied. And spore yield,heat resistance,antibiotic sensitivity,artificial gastrointestinal tolerance,bile salt tolerance and other biological characteristics of Brevibacillus brevis FJAT-1501-BPA as a probiotic strain were evaluated. The results showed that the colloidal suspension agent of Brevibacillus brevis FJAT-1501-BPA was composed of 4% NaCl,0.1‰ calcium citrate,0.1‰ sodium diacetate,0.1‰ potassium sorbate and 2.0‰ xanthan gum with pH 7.Brevibacillus brevis FJAT-1501-BPA could inhibit the growth of Salmonella, Escherichia coli and Staphylococcus aureus. It had good tolerance to artificial gastric and intestinal juice, 0.3% pig bile salt, high spore production rate and strong heat resistance,and it could be used in conjunction with streptomycin and ampicillin. Therefore,Brevibacillus brevis FJAT-1501-BPA could be used as a probiotic strain in livestock production.

Key words: Brevibacillus brevis; artificial gastric juice; artificial intestinal fluid; microbial agent; antibiotic

CLC Number:

S816.7

CHE Jian-mei, LIU Bo, LIU Guo-hong, CHEN Qian-qian, GE Ci-bin, LAN Jiang-lin, YE Shao-wen. Preparation of Brevibacillus brevis FJAT-1501-BPA and Its Evaluation as Probiotic[J]. , 2017, 44(10): 2897-2907.

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Preparation of Brevibacillus brevis FJAT-1501-BPA and Its Evaluation as Probiotic

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