鸡蛋壳蜡样芽孢杆菌的分离鉴定及耐药性分析

doi:10.16431/j.cnki.1671-7236.2022.11.039

Abstract

Abstract: 【Objective】 This study was aimed to analyze the presence of Bacillus cereus on eggs in different environments and the basic characteristics of these isolated strains.【Method】 9 kinds fresh eggs from different farms and supermarkets were collected.Isolation and identification, genotyping, virulence gene screening and drug resistance analysis of Bacillus cereus carried on the surface of eggs were carried out.Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) was used for preliminary identification of isolated strains.The strains were identified, genotyped and screened for virulence genes and resistance genes after whole genome sequencing.The resistance of the strains to 12 antibiotics including ceftriaxone, erythromycin, vancomycin, etc., was tested by the microbroth method.【Result】 After the preliminary identification by MALDI-TOF MS and genomic strain identification, it was determined that 6 strains isolated from 3 kinds of unsterilized fresh eggs in the farms were all Bacillus cereus. The sequence typing (ST) of 6 strains were various, and two new ST types appeared.Non hemolytic enterotoxin gene nheB, diarrheal type virulence factor gene HlyⅡ and immune system invader gene inhA were detected in all 6 strains of Bacillus cereus.The carrying rates of nheA and nheC genes were both 83.3%, and the carrying rates of hblA, hblC and hblD genes which encoded hemolysin BL (HBL) were all 66.7%.The carrying rates of cytK and hlyⅢ genes were 33.3% and 50.0%, respectively.While the enterotoxin genes BceT and entFM and cereulide gene ces were not detected.All of 6 strains were sensitive to gentamicin, tetracycline, chloramphenicol, rifampicin, linezolid and ciprofloxacin, and they were resistant to ampicillin, ceftriaxone and tiamulin.The mediations to erythromycin and clindamycin were 83.3% and 100%, respectively, and 33.3% of strains were resistant to vancomycin.All 6 strains carried the fosfomycin resistance genes FosB and vanRSYZ, part of the vancomycin resistance gene cluster vanA.5 strains carried the macrolide drug resistance gene mphL, and some strains carried the β-lactam drug resistance gene or nucleoside drug resistance gene.【Conclusion】 In this experiment, Bacillus cereus strains were successfully isolated from the shells of unsterilized eggs in the farms.It was found that the isolated strains carried a variety of virulence genes and had multiple drug resistance, with potential pathogenicity and public safety risks.

Key words: egg; Bacillus cereus; virulence genes; antibiotic resistance

CLC Number:

S852.61^＋6

ZHANG Su, FANG Yuwen, CHEN Yunsheng, GAO Li, XU Guiyun, ZHU Kui, ZHENG Shijun, WANG Yongqiang. Isolation,Identification and Antibiotic Resistance Analysis of Bacillus cereus on Eggshell[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4476-4484.

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Isolation,Identification and Antibiotic Resistance Analysis of Bacillus cereus on Eggshell

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