广西罗非鱼无乳链球菌的分离鉴定、毒力基因及耐药性研究

doi:10.16431/j.cnki.1671-7236.2021.07.037

Abstract

Abstract: The purpose of this study was to determine the serotype distribution,virulence gene carrying and drug resistance of Streptococcus agalactiae (S.agalactiae) isolated from tilapia in Guangxi,China,and laid a foundation for the prevention and control of S.agalactiae.47 strains of S.agalactiae were isolated from tilapia farms in Liuzhou,Qinzhou,Nanning and Beihai of Guangxi in 2018-2019,the serotype,distribution of virulence gene and drug resistance were detected and analyzed.The results of serotype test showed that 47 clinical isolates of S.agalactiae strains were Ⅰa serotype.The results of virulence gene detection showed that all clinical isolates of S.agalactiae carried cylE,sodA and gapC genes.The scpB gene was only detected in human reference strain 2603V/R,but not detected in all isolates of piscine S.agalactiae.The results of drug sensitivity test showed that the drug resistance rate of all the piscine isolates to sulfamethoxazole,neomycin,gentamicin and kanamycin was more than 90%,the drug sensitivity of the 47 piscineisolates to of loxacin,levofloxacin,ampicillin,amoxicillin,cefaclor,ceftriaxone,cefoperazone,cefradine,terramycin and doxycycline was 100%.All piscine strains had multiple drug resistance,and 93.62% of the 47 strains were resistanted to more than 5 kinds of drugs.Among all the piscine strains,the isolates resistanted to 9 drugs were accounted for 19.15% and isolated from Liuzhou.The results showed that the serotype of S.agalactiae isolated from tilapia in Guangxi was Ⅰa serotype.All 47 piscine isolates of S.agalactiae carried multiple virulence genes,and the phenomenon of multiple drug resistance was serious.

Key words: Streptococcus agalactiae; tilapia; serotype; virulence gene; antibiotic resistance

CLC Number:

S941.42

GUO Changming, CHEN Huaijun, YUAN Cheng, LUO Fuguang, HUANG Desheng, JIA Guohua, YUAN Sheng, ZHU Shanyuan. Isolation,Identification,Virulence Genes and Antibiotic Resistance Analysis of Streptococcus agalactiae from Tilapia in Guangxi[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(7): 2607-2616.

References

[1] 李新.不同植物脂肪源对吉富罗非鱼生长、组织脂肪酸和生理生化指标的影响[D].武汉:华中农业大学,2016. LI X.Effect of dietary vegetable oils on growth,tissue fatty acid composition and physiological and biochemical index of genetically improved farmed tilapia (Oreochromis niloticus)[D].Wuhan:Huazhong Agricultural University,2016.(in Chinese)
[2] 张德锋,袁伟,可小丽,等.中国罗非鱼主养区无乳链球菌的分子流行特征及其传播方式[J].中国水产科学,2017,24(3):606-614. ZHANG D F,YUAN W,KE X L,et al.Molecular characteristics and transmission of Streptococcus agalactiae in a major tilapia culturing area of China[J].Journal of Fishery Sciences of China,2017,24(3):606-614.(in Chinese)
[3] 梁静真,黄立春,韦慕兰,等.广西罗非鱼源无乳链球菌耐药性及其四环素耐药基因检测[J].南方农业学报,2018,49(10):2077-2086. LIANG J Z,HUANG L C,WEI M L,et al.Detection of antibiotics resistance and tetracycline resistance genes in Streptococcus agalactiae from tilapia in Guangxi[J].Journal of Southern Agriculture,2018,49(10):2077-2086.(in Chinese)
[4] 衣萌萌,王淼,李忠徽,等.无乳链球菌感染对吉富罗非鱼行为的影响及其机制[J].农业生物技术学报,2019,27(5):799-808. YI M M,WANG M,LI Z H,et al.The impacts and mechanism of Streptococcus agalactiae on behavior of Gift tilapia (Oreochromis niloticus),2019,27(5):799-808.(in Chinese)
[5] MHALU F S.Infection with Streptococcus agalactiae in a London hospital[J].Journal of Clinical Pathology,1976,29(4):309-312.
[6] LAKEW B T,FAYERA T,ALI Y M,et al.Risk factors for bovine mastitis with the isolation and identification of Streptococcus agalactiae from farms in and around Haramaya district,Eastern Ethiopia[J].Tropical Animal Health and Production,2019,51(6):1507-1513.
[7] VANDAMME P,DEVRIESE L A,POT B,et al.Streptococcus difficile is a nonhemolytic group B,type Ⅰb Streptococcus[J].International Journal of Systematic Bacteriology,1997,47(1):81-85.
[8] ZAMRI S M,AMAL M N,SITI Z A,et al.Control and prevention of Streptococcosis in cultured tilapia in Maaysia:A review[J].Pertanika Journal of Tropical Agricultural Science,2014,37(4):389-410.
[9] 黄木珍,黎炯,潘忠超,等.宝石鲈无乳链球菌的分离鉴定及耐药性分析[J].中国畜牧兽医,2014,41(10):247-251. HUANG M Z,LI J,PAN Z C,et al.Isolation,identification and drug susceptibility analysis of Streptococcus agalactiae in Scortumbarcoo[J].China Animal Husbandry & Veterinary Medicine,2014,41(10):247-251.(in Chinese)
[10] 黎源,王蓓,汪志文,等.罗非鱼无乳链球菌传播途径与逃避宿主免疫防御策略[J].广东农业科学,2017,44(7):132-140. LI Y,WANG B,WANG Z W,et al.Transmission routes of Streptococcus agalactiae and the strategy of immune evasion in tilapia[J].Guangdong Agricultural Sciences,2017,44(7):132-140.(in Chinese)
[11] 李莉萍,王瑞,黄婷,等.2007-2012年中国罗非鱼无乳链球菌流行菌株血清型分析[J].大连海洋大学学报,2014,9(5):469-475. LI L P,WANG R,HUANG T,et al.Serotype of Streptococcus agalactiae isolated from Nile tilapia Oreochromis niloticus in China from 2007 to 2012[J].Journal of Dalian Ocean University,2014,9(5):469-475.(in Chinese)
[12] LI L P,WANG R,LIANG W W,et al.Rare serotype occumrence and PFGE genotypic diversity of Streptococcus agalactiae isolated from tilapia in China[J].Veterinary Microbiology,2013,167(3-4):719-724.
[13] 胡安东,张明洋,张飘,等.鲤源嗜水气单胞菌分离鉴定、耐药性及毒力基因检测[J].中国畜牧兽医,2019,46(7):2167-2175. HU A D,ZHANG M Y,ZHANG P,et al.Isolation,identification,drug resistance and virulence genes detection of Aeromonas hydrophila from carp[J].China Animal Husbandry & Veterinary Medicine,2019,46(7):2167-2175.(in Chinese)
[14] MOXON E R,KROLL J S.The role of bacterial polysaccharide capsules as virulence factors[J].Current Topics in Microbiology and Immunology,1990,150:65-85.
[15] 祝璟琳,杨弘.鱼源无乳链球菌致病机理研究进展[J].广东海洋大学学报,2013,33(6):92-96. ZHU J L,YANG H.Review on pathogenesis of Streptococcus agalactiae from fish[J].Journal of Guangdong Ocean University,2013,33(6):92-96.(in Chinese)
[16] BOHNSACK J F,TAKAHASHI S,HAMMITT L,et al.Genetic polymorphisms of group B Streptococcus scpB alter functional activity of a cell-associated peptidase that inactivates C5a[J].Infection and Immunity,2000,68(9):5018-5025.
[17] 邓永强,汪开毓.鱼类无乳链球菌病的研究进展[J].中国畜牧兽医,2016,43(9):2490-2495. DENG Y Q,WANG K Y.Research progress on fish Streptococcus agalactiae disease[J].China Animal Husbandry & Veterinary Medicine,2016,43(9):2490-2495.(in Chinese)
[18] BARKHAM T,ZADOKS R N,AZMAI M N A,et al.One hypervirulent clone,sequence type 283,accounts for a large proportion of invasive Streptococcus agalactiae isolated from humans and diseased tilapia in Southeast Asia[J].PLoS Neglected Tropical Diseases,2019,13(6):e0007421.
[19] MARTINEZ G,HAREL J,GOTTSCHALK M.Specific detection by PCR of Streptococcus agalactiae in milk[J].Canadian Journal of Veterinary Research,2001,65(1):68-72.
[20] BAKER G C,SMITH J J,COWAN D A.Review and re-analysis of domain-specific 16S primers[J].Journal of Microbiological Methods,2003,55(3):541-555.
[21] IMPERI M,PATARACCHIA M,ALFARONE G,et al.A multiplex PCR assay for the direct identification of the capsular type (Ⅰa to Ⅸ) of Streptococcus agalactiae[J].Journal of Microbiological Methods,2010,80(2):212-214.
[22] 刘广锦.无乳链球菌鱼源株的免疫原性蛋白筛选和比较基因组学分析[D].南京:南京农业大学,2013. LIU G J.Screening immunogenic proteins and comparative genomics analysis of piscine Streptococcus agalactiae[D].Nanjing:Nanjing Agricultural University,2013.(in Chinese)
[23] 王瑞.无乳链球菌跨宿主感染机制的比较基因组学研究[D].南宁:广西大学,2018. WANG R.Comparative genomic study of mechanisms of cross host infection in Streptococcus agalactiae[D].Nanning:Guangxi University,2018.(in Chinese)
[24] 陈福艳,黎建斌,欧阳贤华,等.鱼鸭混养池塘罗非鱼无乳链球菌病的病原分离鉴定及其防治[J].南方农业学报,2019,50(11):2583-2591. CHEN F Y,LI J B,OUYANG X H,et al.Isolation,identification and prevention of the pathogen ofStreptococcus agalactiae disease in tilapia of fish-duck polyculture pond[J].Journal of Southern Agriculture,2019,50(11):2583-2591.(in Chinese)
[25] DENG L S,LI Y J,GENG Y,et al.Molecular serotyping and antimicrobial susceptibility of Streptococcus agalactiae isolated from fish in China[J].Aquaculture,2019,510:84-89.
[26] ANUBHA S,CAROLIN K,LARA H,et al.The β-hemolysin and intracellular survival of Streptococcus agalactiae in human macrophages[J].PLoS One,2013,8(4):e60160.
[27] MARINA B,MARTA V,VANESSA M,et al.Streptococcus agalactiae GAPDH is a virulence-associated immunomodulatory protein[J].The Journal of Immunology,2007,178(3):1379-1387.
[28] 祝璟琳,季桓涛,肖炜,等.鱼腥草对无乳链球菌引起吉富罗非鱼肝脏损伤的修复作用[J].水产学报,2020,44(7):1187-1197. ZHU J L,JI H T,XIAO W,et al.Protective effects from Houttuyniacordata against Streptococcus agalactiae induced liver injury of Oreochromis niloticus GIFT strain[J].Journal of Fisheries of China,2020,44(7):1187-1197.(in Chinese)

Isolation,Identification,Virulence Genes and Antibiotic Resistance Analysis of Streptococcus agalactiae from Tilapia in Guangxi

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	SHI Jingu, HUANG Shanshan, HUANG Jiwei, CHEN Kunhai, HU Dasheng, ZHU Zhixiong, HAN Shuyu. Comparative Transcriptome Analysis of Streptococcus agalactiae from Tilapia Under High Temperature Stress [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2207-2216.
[2]	CAO Qi, JIN Qing, YUAN Yi, FENG Yixue, KONG Danni, CHEN Xianghong, WU Bin, SONG Wenbo, TANG Xibiao. Epidemiological Investigation of Common Respiratory Bacterial Pathogens in Large-scale Pig Farms [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2439-2449.
[3]	CHEN Jianshi, ZHANG Ning, PU Fangxiong, MIAO Jianjia, ZENG Nan, LI Bingxue. Prediction of Bacillus anthracis Carrying Prophages and Analysis of Drug Resistance and Virulence [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2573-2584.
[4]	WANG Xueping, ZHANG Xiaojun, LI Xiaoyue, WANG Guodong, ZHANG Fuliang, SONG Yuwei, ZHANG Mingliang, MA Lei. Prokaryotic Expression of Hexon Protein of Highly Pathogenic Fowl Adenovirus Serotype 4 and Preparation of Polyclonal Antibodies [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2053-2060.
[5]	LIU Caixia, SHI Weidi, KOU Lijun, MA Xun, WANG Jing, GAO Shengjie, LYU Shuangfei, CHEN Xuke, ZENG Dongdong, KONG Cuilian, REN Huijie. Regulation of Transcription of Key Virulence Factors of Listeria monocytogenes by LIPI-4 EⅡC [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1340-1351.
[6]	WANG Lucai, SALANG Wenzhu, ZHANG Huanrong. Isolation,Identification and Drug Resistance and Pathogenicity Analysis of Salmonella Enteritidis from Avian [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1663-1674.
[7]	WU Zhi, ZHANG Cong, YUAN Huisha, DONG Hongyan, DAI Lixia, JIA Luqi, WU Shuang, ZHU Shanyuan. Isolation,Identification,Virulence and Drug Resistance of Salmonella from Yellow Chicken [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1695-1706.
[8]	ZHU Xihui, LI Yanlan, WANG Zhiwei, PANG Zheyu, LI Yubao, LIU Cheng, SI Zhenshu, LU Jianbiao, CAO Shengliang, XUE Xijuan. Whole Genome Sequencing of a Resistant Riemerella anatipestifer Strain and Analysis of Antibiotic Resistance and Genetic Evolution [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1140-1149.
[9]	CHEN Dong, ZHANG Yanhao, ZHANG Yang, LI Sen, ZHOU Zutao, LI Zili. Isolation,Identification and Drug Sensitivity of 3 Strains of Riemerella anatipestifer [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1250-1258.
[10]	LUO Gan, ZOU Hong, REN Shaoke, WU Chunxia, ZOU Yao, LOU Yinying, ZHOU Yang, CHENG Fangjun, GUO Jianhua. Detection of O-antigen,Virulence Gene and Drug Sensitivity Test of Pathogenic Escherichia coli from Duck [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 713-721.
[11]	YUAN Cheng, CHEN Huaijun, YUAN Sheng, WEN Huamao, CHEN Zhiyi, DONG Hongyan, FENG Qi, GUO Changming. Isolation, Identification and Study on Virulence Gene and Drug Resistance of Streptococcus agalactiae from Tilapia in Zhangzhou City, Fujian Province [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3655-3664.
[12]	WANG Zhifang, XU Yindi, ZHU Wenhao, ZHANG Qingxian, JIAO Wenqiang, LI Haili, WANG Keling. Analysis of Virulence and Drug Resistance of Haemophilus parasuis Serotype 7 Strains [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3245-3255.
[13]	LI Qianqian, LONG Yunzhi, LIANG Gong, JIN Qing, LIU Jinjin, YANG Liu, HUANG Ying, YU Daobing, SONG Wenbo, HUANG Chao, TANG Xibiao. Screening of Vaccine Candidate Strains of Streptococcus suis Serotype 3 and Evaluation of the Immune Effect of the Bivalent Inactivated Vaccine Streptococcus suis Serotype 3+9 on Mice [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2307-2317.
[14]	WANG Simin, YANG Jiangfeng, ZHAO Xiaokun, ZHANG Lei, GUO Jingjing, LAN Jinping, YU Xiaojie, WANG Jing. Isolation, Identification and Analysis of Pathogenicity and Drug Resistance of Salmonella from Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2385-2396.
[15]	DONG Hongyan, SONG Haigang, ZHU Shanyuan, YU Shengqing, XIE Jun, HONG Shuangpeng, GUO Changming, XU Hai, GAO Yuexiu. Isolation,Identification and Pathogenicity Analysis of Escherichia coli from Ducks [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1977-1984.