中国美利奴羊OLA-DQB1基因exon2遗传多态性与布鲁氏菌病易感性的关联分析

doi:10.16431/j.cnki.1671-7236.2015.06.026

Abstract

Abstract: The single nucleotide polymorphisms (SNPs) of ovine lymphocyte antigen DQB1 (OLA-DQB1) gene exon 2 was amplified by PCR-SSCP method from 148 healthy and 60 infected with Brucella Chinese Merino sheep and then PCR products of different alleles were sequenced to determine the polymorphism loci of the gene.The differences in gene frequency and genotype frequency of each SNP loci were analyzed statistically to analyze its correlation with brucellosis susceptibility.The sequencing result showed that 43 SNPs were detected in 270 bp DNA sequence,the gene frequencies of G196A allele had extremely significant difference in case and control samples (P< 0.01),and its genotype frequencies presented significant difference (P< 0.05).Similarly,C211T allele was significantly different in case and control samples (P< 0.05).The results showed that the polymorphism of OLA-DQB1 gene exon 2 might be a significant association gene with brucellosis susceptibility.

Key words: PCR-SSCP; Chinese Merino sheep; OLA-DQB1 gene; single nucleotide polymorphisms (SNPs); brucellosis susceptibility

CLC Number:

S813.3

WANG Wen-wen, XU Wan-yun, HU Meng-wei, LI Jian-hua, LIU Peng-tao, GAO Jian-feng. The Correlation Analysis between Chinese Merino Sheep OLA-DQB1 Gene Exon 2 Genetic Polymorphism and Brucellosis Susceptibility[J]. , 2015, 42(6): 1495-1503.

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The Correlation Analysis between Chinese Merino Sheep OLA-DQB1 Gene Exon 2 Genetic Polymorphism and Brucellosis Susceptibility

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