鹌鹑野灰相关蛋白基因外显子Ⅰ的多态性分析

doi:10.16431/j.cnki.1671-7236.2017.05.024

Abstract

Abstract:

The poultry feather color inheritance has been a hot research topic, because the feather color is a kind of important quality character.In this study, we used polyacrylamide gel electrophoresis to detect the polymorphisms of four quail populations of different feather color (China Yellow quail, Black quail, Korean quail, Beijing White quail) of agouti-related protein gene (Agrp) exon Ⅰ, so as to investigate the relationship of Agrp exon I with quail plumage color, and to provide reference for the breeding and production of quail.The results showed that two alleles were detected in the Agrp exon Ⅰ of China Yellow quail, Black quail, Korean quail, Beijing White quail populations,and expressed by A and B, respectively. Frequency of allele A in Beijing White quail was up to 0.9500. The frequency of B allele in Korean quail was the highest, reaching 0.3392, in Beijing White quail genes in the lowest frequency is only 0.0500. The genotype frequency of AA genotype in Beijing White quail was the highest (0.9500), the lowest in Korean quail and Black quail (0.4286). BB genotype was not observed in Beijing White quail. We speculated that it may have a certain relationship between Agrp exon Ⅰ B allele and feather color of quail.

Key words: quail; agouti-related protein gene(Agrp); PCR-SSCP

CLC Number:

S839.2

BAI Jun-yan, YANG Shuai, PANG You-zhi, WANG Xu, SUN Gang. Polymorphism Analysis of the Agouti-related Protein Gene Exon Ⅰof Quail[J]. , 2017, 44(5): 1425-1430.

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Polymorphism Analysis of the Agouti-related Protein Gene Exon Ⅰof Quail

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