黑羽鹌鹑的EST-SSR标记开发与应用

doi:10.16431/j.cnki.1671-7236.2016.05.030

Abstract

Abstract: The purpose of this study was to accelerate the application of molecular markers in genetic breeding and other aspects of Black quail to develop EST-SSR markers for Black quail.100 Black quail were randomly chosen and blood samples were collected to develop EST-SSR new markers and determined the genetic polymorphism using bioinformatics.The results showed that there were 6 EST-SSR markers in the Black quail, the number of observed alleles in EST-SSR was 2 to 6.The average PIC of 6 EST-SSR markers in the Back quail was 0.4962 and the average He was 0.5759.Markers P1 and P2 were highly polymorphic markers among 6 EST-SSR and the rest EST-SSR markers were moderately polymorphic markers.These developed markers could be used for genetic diversity analysis of Black quail and the EST-SSR markers had some relationship with the carbohydrate metabolism, synthesis of melanin in Black quail, but the mechanism needed the further analysis.

Key words: Black quail; EST-SSR; bioinformatics; genetic diversity

CLC Number:

S839.2

BAI Jun-yan, PANG You-zhi, YANG You-bing, QI Yan-xia, LI Hong-wei. Development and Application of EST-SSR Markers of Black Quail[J]. , 2016, 43(5): 1323-1328.

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Development and Application of EST-SSR Markers of Black Quail

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