3个鹌鹑群体的微卫星标记多态性分析

doi:10.16431/j.cnki.1671-7236.2015.09.034

Abstract

Abstract: In order to reveal the genetic structure and genetic relationship of Korean quail, Chinese Yellow quail, Chinese Black quail, the 3 quail populations from the molecular level, to provide for the study of genetic resources of quail.This study used PCR amplification, polyacrylamide gel electrophoresis, cluster analysis and other methods of 3 quail populations were polymorphic analysis of microsatellite markers.The results showed that 12 microsatellite markers in 3 quails was detected in the observed number of alleles was between 4 and 7, the average polymorphism information content of Chinese Yellow quail, Chinese Black quail, Korean quail were 0.6853, 0.6401 and 0.6565, respectively;The average heterozygosity were 0.7333, 0.6957 and 0.7111, respectively, which Chinese Yellow quail population genetic diversity was the most abundant polymorphism.The cluster analysis showed that the Chinese Black quail and Korean quail smallest genetic distance was 0.0628, so the Chinese Black quail and Korean quail were clustered into one category, and then was Chinese Yellow quail together.This was further evidence of Chinese Black quail and Korean quail had a closer genetic relationship, and the genetic relationship of Chinese Black quail and Korean quail was more distant with Chinese Yellow quail.

Key words: quail; microsatellite marker; polymorphism; genetic diversity

CLC Number:

S839.2

BAI Jun-yan, PANG You-zhi, ZHANG Xiao-hui, YUN Yin-xian, ZHAO Shu-juan, QI Yan-xia. Polymorphism Analysis of 3 Quail Groups by Using Microsatellite Marker[J]. , 2015, 42(9): 2444-2452.

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Polymorphism Analysis of 3 Quail Groups by Using Microsatellite Marker

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