黑羽鹌鹑ESRβ基因外显子多态性及其与产蛋性状的相关性分析

doi:10.16431/j.cnki.1671-7236.2016.12.031

Abstract

Abstract:

This study aimed to investigate the correlation between ESRβ gene exon polymorphism and egg production of Black plumage egg quail, and provide the theoretical foundation for molecular selection. The healthy Black plumage egg quail were selected as research object, and the egg production were analyzed using SPSS 13.0 software. Estrogen receptor β(ESRβ) subunit gene was selected as candidates gene to screen the genomic variation by PCR-SSCP, and the association of the ESRβ gene polymorphisms with egg production were analyzed. The results of polymorphisms detection showed that exon 1, exon 3, exon 4, exon 5,exon 6,exon 7 and exon 8 of ESRβ gene all had nucleotide variation. The association analysis results of ESRβ gene exons and egg production showed that the egg weight of first laying of exon 3 CD genotype were significantly higher than that of CC and DD genotypes (P<0.05). The age at first laying of exon 5 was also significant difference between DD and CC, CD genotypes (P<0.05). Moreover, the average egg weight of exon 7 CD genotype was significant higher than that of CC and DD genotypes (P<0.05). These data suggested that ESRβ gene could significantly affect egg production of Black plumage egg.

Key words: quail; ESRβ gene; exon; SNPs; egg production

CLC Number:

S813.3

XIE San-lei, WEN Feng-yun, LI Jun-qiang, WANG Qing, LIANG Yu, LIU Yi-ting, LI Lei, CHEN Yi. Single Nucleotide Polymorphisms in ESRβ Gene of Black Plumage Quail and Its Association with Egg Production[J]. , 2016, 43(12): 3293-3299.

References

[1] 程瑛琨,鄂晨光,刘明石,等.鸡蛋、乌鸡蛋、鹌鹑蛋营养成分的测定比较[J].饲料工业,2005,26(7):10-12.
[2] 赵国燕.吃香的鹌鹑蛋[J].农产品加工,2009,6:32.
[3] 皮劲松,杜金平,申杰,等.鹌鹑研究进展[J].安徽农业科学,2007,35(10):2926-2927.
[4] 彭勇博,杜金平,申杰,等.黄羽鹌鹑产蛋性状遗传参数的估计[J].安徽农业科学,2010,38(2):742-743.
[5] Masaoke-Tsudzuke,陈蓉,秦玉蓉.鹌鹑形态学和行为学突变以及分子遗传研究进展[J].中国家禽,2009,31(17):47-51.
[6] 付晶,孙洪霞,白秀娟.鹌鹑性别鉴定的分子标记方法[J].东北农业大学学报,2010,41(1):77-80.
[7] 王旭,白俊艳,张小辉,等.不同鹌鹑群体EST-SSR标记多态性分析[J].中国家禽,2016,38(3):46-48.
[8] Benita S. Estrogen receptor:Bioactivities and interactions with cell signaling pathways[J].Biology of Reproduction,1996,54:287-293.
[9] Rothschild M, Jacobson C, Vaske D, et al. The estrgen receptor locus is associated with a major gene influencing litter size in pigs[J]. Proceedings of the National Academy of Science,1996, 93(1):201-205.
[10] Kuiper G G, Enmark E, Pelto-Huikko M, et al. Cloning of a novel receptor expressed in rat prostate and ovary[J]. Proceedings of the National Academy of Sciences,1996,93(12):5925-5930.
[11] 白俊艳,庞有志,张小辉,等.3个鹌鹑群体的微卫星标记多态性分析[J].中国畜牧兽医,2015,42(9):2444-2452.
[12] 庞有志,白俊艳,张小辉,等. 北京白羽鹌鹑微卫星多态性分析[J].江苏农业科学,2015, 31(5):1110-1114.
[13] 吴胜军,庞有志,赵淑娟,等. 朝鲜鹌鹑微卫星多态性分析[J].河南农业科学,2010,10:112-115.
[14] 唐立群,肖层林,王伟平.SNP分子标记的研究及其应用进展[J].中国农学通报,2012,28(12):154-158.
[15] 史亚兴,卢柏山,宋伟, 等. 基于SNP标记技术的糯玉米种质遗传多样性分析[J]. 华北农学报,2015, 30(3):77-82.
[16] 曲娟娟,于敏,董蕾,等.SNP在动植物遗传育种中的应用[J].东北农业大学学报,2011,42(12):9-13.
[17] 康静婷,梁前进.单核苷酸多态性的研究及其生物学意义[J].生物学通报,2012,47(3):15-18.
[18] Palshof T,Blichert-Toft M,Daehnfeldt J L, et al. Estradiol binding protein in cystosarcoma phyllodes of the breast[J]. European Journal of Cancer, 1980,16(5):591-593.
[19] Green S,Walter P,Kumar V, et al.Human estrogen receptor cDNA:Sequense,expression and homology to v-erb-A[J]. Nature, 1986, 320(13):134-139.
[20] 陈克飞,黄路生,李宁,等.猪雌激素受体(ESR)基因对产仔数性状的影响[J].遗传学报,2000,27(10):853-857.
[21] 徐宁迎,章胜乔,彭淑红.金华猪3个繁殖性状主基因的分布及其效应的研究[J].遗传学报,2003,30(12):1090-1096.
[22] Wu Z F, Liu D W, Wang Q L, et al. Study on the association between estrogen receptor gene (ESR) and reproduction traits in Landrace pigs[J]. Acta Genetica Sinica, 2006, 33(8):711-716.
[23] 毕晓丹,储明星,金海国,等.小尾寒羊高繁殖力候选基因ESR的研究[J].遗传学报,2005,32(10):1060-1065.
[24] 周俊,虞德病,洪坤月,等. 鸡FSHβ和ESRβ基因SNPs分析与早期产蛋性能关系[J].华北农学报,2008,23(5):49-52.
[25] 王洪才,宋天玉,袁晓春. 庄河大骨鸡ESR基因多态性及其与产蛋性能关联的分析研究[J]. 中国农学通报,2012,28(20):43-46.
[26] 于吉英,陈宽维,肖小君,等.ESR、NPY基因对文昌鸡繁殖性状的遗传效应分析[J].畜牧与兽医,2008,40(4):49-51.
[27] 汤青萍,朱文奇,吴旭,等.ESR基因与文昌鸡产蛋性状的关联性研究[J].云南农业大学学报,2009,24(1):67-70.

Single Nucleotide Polymorphisms in ESRβ Gene of Black Plumage Quail and Its Association with Egg Production

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