牦牛和犏牛促卵泡素受体基因5'-侧翼区序列多态性研究

doi:10.16431/j.cnki.1671-7236.2015.04.031

Abstract

Abstract: In this study, the genetic polymorphism of 5'-flank region of follicle-stimulating hormone receptor (FSHR) gene in yaks and cattle-yak were detected in order to offer the materials and theoretical foundation for solving the problem of lower yak's reproduction rate and screening the molecular markers for reproductive traits. The polymorphism of the partial 5'-flank region of the FSHR gene in 110 individuals from Maiwa yaks, Jiulong yaks, Datong yaks and cattle-yak was analyzed by PCR-SSCP. The parameters of alleles frequency, genotype frequency, linkage equilibrium of Hardy-Weinberg, heterozygosity (H), effective numbers of alleles (Ne) and polymorphism information content (PIC) were calculated by statistical methods. The results showed that the 5'-flank region of FSHR gene in Maiwa yak, Datong yak and Jiulong yak had polymorphism, but no polymorphism found in cattle-yak. There were three genotypes (AA, AB and BB) in Maiwa yak, two genotypes (AA and AB) in Jiulong yak and Datong yak, AB genotype was favorable genotype in three yak breeds, and A allele was favorable allele. The polymorphism information content were 0.3693, 0.3565 and 0.3705 in Maiwa yak, Jiulong yak and Datong yak, respectively, which reached a moderate polymorphism (0.25<PIC<0.5). It indicated that there was greater genetic variation in all yak breeds.

Key words: yak; cattle-yak; FSHR gene; PCR-SSCP

CLC Number:

S823.8⁺5

CHEN Sheng-mei, LUO Hong, NIU Jian-zhang, MA Yu-hong, CHAI Zhi-xin, MA Zhi-jie, ZHONG Jin-cheng. Study on Polymorphism of 5'-Flank Region of FSHR Gene in Yak and Cattle-yak[J]. , 2015, 42(4): 980-985.

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Study on Polymorphism of 5'-Flank Region of FSHR Gene in Yak and Cattle-yak

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