中国美利奴羊eNOS基因exon8多态性及其与布鲁氏菌病易感性相关性分析

doi:10.16431/j.cnki.1671-7236.2015.12.027

Abstract

Abstract: The association of Chinese Merino sheep eNOS gene exon8 polymorphisms with brucellosis susceptibility was studied in this research.The sequences of human and Chinese Merino sheep eNOS gene exon8 were aligned by the bioinformatics methods,and the polymorphisms of human eNOS gene in the NCBI SNP database were statistically analyzed.The polymorphisms of 101 Chinese Merino sheep negative samples and 61 Chinese Merino sheep positive samples were detected by PCR-SSCP method,and then the PCR products of different alleles were sequenced,aiming to determine exon8's polymorphism loci,and the allele frequency and genotype frequency of SNP loci were statistically analyzed.A novel SNP locus (ss974768653:A142G) was detected at the 142 bp of eNOS gene exon8,and the locus had no difference in allele frequency and each genotype between negative and positive groups (P >0.05).There might be no correlation between the A142G polymorphism locus of Chinese Merino sheep eNOS gene exon8 and brucellosis susceptibility.

Key words: sheep; eNOS; PCR-SSCP; polymorphism; exon

CLC Number:

Q754

XU Wan-yun, WANG Hui-min, LI Jian-hua, WANG Wen-lun, HU Meng-wei, GAO Jian-feng. The Polymorphisms of Chinese Merino Sheep eNOS Gene exon8 and its Association with Brucellosis Susceptibility[J]. , 2015, 42(12): 3294-3299.

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The Polymorphisms of Chinese Merino Sheep eNOS Gene exon8 and its Association with Brucellosis Susceptibility

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