广西南宁市水牛肝脏内前后盘吸虫的鉴定

doi:10.16431/j.cnki.1671-7236.2021.02.029

Abstract

Abstract: To identify the accurate species of Paramphistomidae in buffalos of Nanning,Guangxi,trematode identification was performed after collected from livers.In this study,the liver of buffaloes in a slaughterhouse in Nanning was dissected and the pathological lesion was recorded.Afterwards,adult worms in the livers were collected and incubated to lay eggs,then morphology of the adult worms and eggs were observed.The adult worms were also stained with hematoxylin-eosin (HE) and hematoxylin to facilitate its internal structure observation.Finaly,adult worms were identified by molecular biological method.The results showed that there were bleeding spots on the infected liver surface.Besides,congestion,purulent focus,liver enlargement and softer texture was also detectable.The eggs were oval,light gray,covered with round embryo cells,and the yolk cells did not fill the whole egg.The adult was composed of body wall,internal reproductive and digestive system.The body wall was composed of cortex and muscular layer.The reproductive system,digestive tract and some muscle bundles and fibrous tissues also could be seen.The reproductive system included the reproductive organs such as ovary,uterus and testis,while the digestive tract was distributed throughout the body cavity.The amplified product of ITS-2 gene was 441 bp,and comparison with Explanatum explanatum (AB743577.1) in GenBank showed that the similarity was 99.3%.Phylogenetic analysis showed its close relationship with Explanatum explanatum,and they were also located in the same branch.Considering the pathological lesion,egg characteristics,adult morphological and molecular biology characteristics,flukes here in our study was determined as Explanatum explanatum. The results of this study provided a theoretical basis for the classification and identification of trematodes,and further enrich the species of this family.In addition,we have a preliminary understanding of the prevalence of Paramphistomidae in buffaloes in Nanning,Guangxi,and provided a theoretical basis for the accurate control of trematode infection.

Key words: egg morphology; HE staining; morphological identification; molecular biological identification; Paramphistomidae

CLC Number:

S855.9

WU Zhengjiao, HOU Linjing, RAO Guoshun, JIN Weikun, FANG Fang, ZHANG Weiyu, DI Wenda. Identification of Paramphistomidae in the Liver of Buffalos in Nanning,Guangxi[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(2): 668-675.

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Identification of Paramphistomidae in the Liver of Buffalos in Nanning,Guangxi

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