威宁绵羊SOCS7和GFAP基因组织表达研究

doi:10.16431/j.cnki.1671-7236.2019.07.003

Abstract

Abstract:

This study was aimed to detect the expression of suppressor of cytokine signaling 7 (SOCS7) and glial fibrillary acidic protein (GFAP) genes in different tissues of Weining sheep.This experiment took male and female Weining sheep aged 6 months,1 year and 2 years as research objects,the relative expression of SOCS7 and GFAP genes in six tissues of heart,liver,spleen,lung,kidney and brain at different sex and growth stages were determined by Real-time quantitative PCR,and the mRNA level expression pattern of SOCS7 and GFAP genes were explored.The results showed that SOCS7 gene was expressed in different tissues of Weining sheep,the expression of spleen was the highest,followed by lung,kidney,heart,liver and brain,and the relative expression of SOCS7 gene in Weining sheep slightly increased with age.The relative expression of GFAP gene was the highest in brain of Weining sheep,while the rest of the tissues showed a low expression level,which showed a downward trend with age.According to the genders difference,the relative expression of SOCS7 gene in female was generally higher than male,while that of GFAP gene in male was generally higher than female,it suggested that SOCS7 gene might negatively regulate the expression of GFAP gene.

Key words: Weining sheep; SOCS7 gene; GFAP gene; Real-time quantitative PCR; tissue expression

CLC Number:

Q786

ZHANG Ji, YI Ming, CHENG Chaoyou, CHENG Junhua, FU Zhengxian, YANG Dewen, WU Xue, QIU Ganyuan, LIU Ruoyu. Study on Tissue Expression of SOCS7 and GFAP Genes in Weining Sheep[J]. , 2019, 46(7): 1899-1906.

References

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Study on Tissue Expression of SOCS7 and GFAP Genes in Weining Sheep

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