猪TLR5基因表达水平与F18大肠杆菌侵染关系分析

doi:10.16431/j.cnki.1671-7236.2018.05.005

Abstract

Abstract:

In order to explore the relationship between Toll-like receptor 5 (TLR5) gene expression and resistance to E.coli F18,the bacterial stimulation experiments were conducted in this study.Intestinal epithelial cells (IPEC-J2) were induced by lipopolysaccharide (LPS) and E.coli F18 with different serotypes (F18ab and F18ac),the mRNA expression level of TLR5 gene was detected by Real-time PCR,and the protein expression was detected by Western blotting.The results showed that the expression level of TLR5 gene was extremely significantly up-regulated (P<0.01) after IPEC-J2 cells were infected with different serotypes of E.coli (F18ab and F18ac),which was also extremely significantly up-regulated after IPEC-J2 cells were induced by LPS for 4 and 8 h (P<0.01).After IPEC-J2 cells were induced by LPS for 8 h,the expression level of TLR5 gene increased more significantly than induction for 4 h.After LPS and bacterial stimulation,the expression level of TLR5 protein in cells was extremely significantly up-regulated (P<0.01),which was consistent with the results of mRNA expression.This study further confirmed that the expression level of porcine TLR5 gene played an important role in the process of cell resistance to E.coli F18 infection by studying the relationship between the expression level of TLR5 gene and E.coli.It also laid the foundation for future research on TLR5 gene function and its application in genetic breeding of E.coli diarrhea.

Key words: porcine; TLR5 gene; E. coli; mRNA expression; Western blotting

CLC Number:

S828

ZHAO Chengxiang, DAI Kaiyu, DU Yulu, BAO Wenbin, WU Shenglong. Analysis of the Relationship Between Expression Level of Porcine TLR5 Gene and Infection of Escherichia coli F18[J]. , 2018, 45(5): 1163-1169.

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Analysis of the Relationship Between Expression Level of Porcine TLR5 Gene and Infection of Escherichia coli F18

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