感染16型蓝舌病病毒后绵羊部分细胞因子消长特点研究

doi:10.16431/j.cnki.1671-7236.2021.01.033

Abstract

Abstract: The purpose of this experiment was to investigate the growth and decline characteristics of IFN-γ,IL-2,IL-4,IL-10 of sheep after infection with bluetongue virus type 16 (BTV16).In this study,the mRNA of the above four cytokines were detected in three sheep infected with BTV16 using the Real-time quantitative PCR method.At the same time,negative control sheep were set up,and the relative expression of mRNA were calculated based on 0 d mRNA.Meanwhile,virus antibody titers were detected and sheep body temperature was measured.The results showed that all the three sheep inoculated with BTV16 had different degrees of antibody and body temperature symptoms,the mRNA levels of the four cytokines increased significantly within 2-4 days after inoculation of the virus.Among them,the peak value of IFN-γ was 2.58-27.84 times,the peak value of IL-2 was 5.24-17.19 times,the peak value of IL-4 was 2.16-3.43 times,and the peak value of IL-10 was 15.78-48.77 times.There were significant individual differences in the increase amplitude,and all the four cytokines decreased gradually after being kept at a high level for about 6 d.The above parameters of control sheep fluctuated within the normal range with no significant difference (P>0.05).This study elucidated the growth and decline characteristics of IFN-γ,IL-2,IL-4 and IL-10 in sheep inoculated with BTV16 at the transcriptional level,providing references for further studies on the characteristics of BTV infection and the immune mechanism of host organism.

Key words: bluetongue virus type 16 (BTV16); cytokine; mRNA; growth and decline characteristics

CLC Number:

S826

MIAO Haisheng, KOU Meiling, XIE Jiarui, LI Le, LI Huachun. Study on the Growth and Decline Characteristics of Some Cytokines in Sheep after Infected with Bluetongue Virus Type 16[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(1): 296-302.

References

[1] JENCKEL M,BREARD E,SCHULZ C,et al.Complete coding genome sequence of putative novel bluetongue virus serotype 27[J].Genome Announcements,2015,3(2):15-16.
[2] GIULIETTI A,OVERBERGH L,VALCKX D,et al.An overview of Real-time quantitative PCR:Applications to quantify cytokine gene expression[J].Methods,2001,25(4):386-401.
[3] BOSURGI L,CAO Y G,CABEZA-CABRERIZO M,et al.Macrophage function in tissue repair and remodeling requires IL-4 or IL-13 with apoptotic cells[J].Science,2017,356(6342):1072-1076.
[4] CASELLA G,GARZETTI L,GATTA A T,et al.IL4 induces IL6-producing M2 macrophages associated to inhibition of neuroinflammation in vitro and in vivo[J].Journal of Neuroinflammation,2016,13(1):139.
[5] MICHAEL J D,MAVIS A A,MA Y G,et al.Exogenous IL-4 shuts off pro-inflammation in neutrophils while stimulating anti-inflammation in macrophages to induce neutrophil phagocytosis following myocardial infarction[J].Journal of Molecular and Cellular Cardiology,2020,145:112-121.
[6] LAN R T.Veterinary Immunology[M].Singapore:Elsevier Pte Ltd,2011.
[7] BOEUF P,VIGAN-WOMAS I,JUBLOT D,et al.CyProQuant-PCR:A Real-time RT-PCR technique for profiling human cytokines,based on external RNA standards,readily automatable for clinical use[J].BMC Immunology,2005,6:5.
[8] MENA A,IOANNOU X P,VAN KESSEL A,et al.Th1/Th2 biasing effects of vaccination in cattle as determined by Real-time PCR[J].Journal of Immunological Methods,2002,263(1-2):11-21.
[9] OVERBERGH L,GIULIETTI A,VALCKX D,et al.The use of Real-time reverse transcriptase PCR for the quantification of cytokine gene expression[J].Journal of Biomolecular Techniques,2003,14(1):33-43.
[10] SATORU K,TATSUFUMI U,KAZUHIKO O,et al.The rapid quantitative analysis of bovine cytokine genes by Real-time RT-PCR[J].Veterinary Microbiology,2003,94:283-294.
[11] 苗海生,李乐,廖德芳,等.蓝舌病多克隆抗体C-ELISA检测方法的建立和比较研究[J].云南农业大学学报(自然科学),2014,29(1):58-62. MIAO H S,LI L,LIAO D F,et al.Research on establishment and comparison of blue-tongue-virus multi-clone antibody C-ELISA[J].Journal of Yunnan Agricultural University(Natural Science),2014,29(1):58-62.(in Chinese)
[12] ESPINO A M,RIVERA F.Quantitation of cytokine mRNA by Real-time RT-PCR during a vaccination trial in a rabbit model of fascioliasis[J].Veterinary Parasitology,2010,169(1-2):82-92.
[13] ABDALLA A O,KIAII S,HANSSON L,et al.Kinetics of cytokine gene expression in human CD4⁺ and CD8⁺ T-lymphocytesubsets using quantitative Real-time PCR[J].Scandinavian Journal of Immuno-logy,2003,58(6):601-606.
[14] AUDIGE A,SCHLAEPFER E,BONANOMI A,et al.HIV-1 does not provokealteration of cytokine gene expression in lymphoid tissue after acute infection ex vivo[J].Journal of Immunology,2004,172:2687-2696.
[15] STEPHENS S A,BROWNLIE J,CHARLESTON B,et al.Differences in cytokine synthesis by the sub-populations of dendritic cells from afferent lymph[J].Immunology,2003,120(1):48-57.
[16] SANCHEZ-CORDOPN P J,PEREZ DE DIEGO A C,GOMEZ-VILLAMANDOS J C,et al.Comparative analysis of cellular immune responses and cytokine levels in sheep experimentally infected with bluetongue virus serotype 1 and 8[J].Veterinary Microbiology,2015,177(1-2):95-105.
[17] SANGEETA B,LISA F H,IAN M,et al.Quantitation of ovine cytokine mRNA by Real-time RT-PCR[J].Journal of Immunological Methods,2006,309(30):160-172.
[18] PAUL W E,ZHU J.How are T(H)2-type immune responses initiated and amplified?[J].Nature Reviews Immunology,2010,10(4):225-235.
[19] FRANTZESKAKI F G,KARAMPI E S,KOTTARIDI C,et al.Cytomegalovirus reactivation in a general,nonimmunosuppressed intensive care unit population:Incidence,risk factors,associations with organ dysfunction,and inflammatory biomarkers[J].Journal of Critical Care,2015,30(2):276-281.
[20] CHANNAPPANAVAR R,PERLMANAN S.Patho-genic human coronavirus infections:Causes and conse-quences of cytokine storm and immunopathology[A].Seminars in Immunopathology,Springer Berlin Heidelberg[C].2017.
[21] LIU Q,ZHOU Y,YANG Z.The cytokine storm of severe influenza and development of immunomo-dulatory therapy[J].Cellular & Molecular Immunology,2016,13(1):3-10.

Study on the Growth and Decline Characteristics of Some Cytokines in Sheep after Infected with Bluetongue Virus Type 16

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	SHAO Yongbin, ZHENG Yichuang, ZHANG Jingyi, WEI Linying, TIAN Yong, SUN Guojie, LIU Yuran, LUO Yan, GU Xinli. Study on the Anti-inflammatory Effect of Leonurine on LPS-induced Endometritis in Mice [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2149-2155.
[2]	LIU Haoyuan, LI Yanjie, QIN Hewei, JIN Xiaoqin, NIU Yuqing, HUA Xiaoqiong, ZHANG Shuqin, NIU Li. Study on the Mechanism of WU Shen Venation Smooth Decoction on Atherosclerosis AopE^-/- Mice Based on Network Pharmacology [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 377-389.
[3]	CHEN Shiyu, CUI Shuai, JIANG Yajun, XIN Ting, WANG Yang, HAO Yuxin, HUANG Jianxin, GUO Xiaoyu, ZHU Hongfei, WU Jiajun, JIA Hong. lncRNA-mRNA Analysis of Toll-like Receptor Signaling in Porcine Peripheral Blood Lymphocytes After African Swine Fever Virus Infection [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2677-2687.
[4]	LIU Yuran, YANG Huiying, ZHU Yan, WANG Mengmeng, SUN Guojie, ZHANG Mengyuan, WANG Yan, GAO Jiahao, LIAN Kexun, GU Xinli. Study on the Anti-inflammatory Effect of ‘Ruyan Ning’ Water Extract [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1879-1887.
[5]	CHEN Yu, CHEN Xiaolan, YANG Haifeng, LUO Jun, ZHANG Ting, ZUO Haiping, ZHENG Yi. Therapeutic Effect of Wuwei Zhili Mixture on Diarrhea Induced by Escherichia coli in Mice [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 2001-2009.
[6]	QIU Shulei, CHEN Xiaolan, YANG Haifeng, CHEN Yuku, HU Yuanliang, GAO Yongxu, WU Caihong. Effects of Selenized Garlic Polysaccharide on the Function of Mouse Peritoneal Macrophages [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1144-1152.
[7]	YIN Huihui, TONG Yanmei, ZENG Xueyan, LU Guangqiang, ZHAO Wu, JIANG Yuanming, LIU Wei. Effect of Polysaccharide from Rhodomyrtus tomentosa Fruit on Immunological Function in Immunosuppressed Mice [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 731-737.
[8]	ZHAO Yi, ZHANG Wen, CHEN Jiaji, SONG Manling, CHEN Qi, XIE Xiaodong, HU Tingjun. Establishment of 3D4/2 Cell Inflammatory Model Induced by PCV2 in vitro [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(12): 4920-4927.
[9]	REN Qiaoling, ZHANG Jiaqing, GUO Hongxia, WANG Xianwei, WANG Jing, LYU Lingyan, CHEN Junfeng, MA Qiang, ZHANG Bin, XING Baosong, XU Zejun. Screening and Analysis of Differentially Expressed Genes in Hypothalamus-Pituitary-Ovary Axis of Estrous and Anestrous Primiparous Sows [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 2864-2877.
[10]	ZHANG Jing, QIAN Yinghong, ZHANG Kai, WU Jindi, LIU Bo, MAO Wei, CAO Jinshan. Effects of Staphylococcus aureus Lipoprotein on Immunity in M1 Mouse Bone Marrow-Derived Macrophages [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 3079-3086.
[11]	LI Botong, WANG Shiyu, LIU Jiawei, ZHANG Weiqi, NI Yaodi, LIU Mingchao. Effect of Escherichia coli on Inflammatory Damage of Bovine Endometrial Epithelial Cells [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(7): 2627-2634.
[12]	SHU Jingting, JI Gaige, TU Yunjie, ZHANG Ming, JU Xiaojun, SHAN Yanju, LIU Yifan, ZOU Jianmin. Tissue Expression Analysis of Wnt3a Gene and Genetic Effects of SNPs on Feather Follicle Density in Chicken [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(5): 1672-1680.
[13]	REN Chunzhi, LU Caixia, WEI Yingyi, YU Meiling, HU Tingjun. Effect of Ethyl Acetate Fraction of Flavonoids from Polygonum hydropiper L. on Cytokines Secretion in RAW264.7 Cells Induced by Pseudorabies Virus [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(3): 1132-1140.
[14]	JIAO Hanwei, ZHOU Zhixiong, LI Mengjuan, XIAO Yu, LI Bowen, GUO Xiaoyi, ZENG Hui, GU Guojing, SHUAI Xuehong. Identification of lncRNA-mRNA Regulatory Network of Riboflavin Metabolism in HepG2 Cells Affected by Swine Hepatitis E Virus ORF3 Protein [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(12): 4618-4627.
[15]	MA Zhanbang, XU Liwen, WANG Fangfang, ZHANG Lili, HOU Yutong, HAN Zongxi, MA Deying. Effects of Different Origins of Infectious Bronchitis Virus on Immune Function in SPF Chicken [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(12): 4628-4640.