非洲猪瘟病毒感染后猪外周血淋巴细胞Toll样受体信号通路的lncRNA-mRNA分析

doi:10.16431/j.cnki.1671-7236.2022.07.026

Abstract

Abstract: 【Objective】 This study was aimed to enrich the long non-coding RNA (lncRNA) expression profile of pig peripheral blood lymphocytes after African swine fever virus (ASFV) infection,and further explore the regulatory network affecting the Toll-like receptor signaling pathway.【Method】 Experimental animals were infected with ASFV, and peripheral blood was collected on the 7th day and isolated to obtain peripheral blood lymphocytes.The lncRNAs in peripheral blood lymphocytes were sequenced by Illumina high-throughput genomic sequencing.The raw data were processed and screened to obtain differentially expressed lncRNA,and target gene prediction was performed.GO function and KEGG signaling pathway enrichment analysis were performed using bioinformatics methods,and preliminary mapping of the lncRNA-mRNA regulatory networks related to the Toll-like receptor signaling pathway and 4 lncRNAs including lncRNA-ENSSSCG00000041959 were verified by Real-time quantitative RT-PCR.【Result】 A total of 73 differentially expressed lncRNAs were screened,including 38 upregulated and 35 downregulated lncRNAs.GO function analysis showed that target genes were significantly enriched in regulating immune system process,defense response,biological response,viral response and innate immunity.KEGG signaling enrichment analysis showed that most target genes were related to cell circulation,disease and immune response,including immune related signaling were Toll-like receptor signaling pathway,TNF signaling pathway,and intestinal immune network producing IgA.lncRNA-ENSSSCG00000041959-RIPK1 and lncRNA-ENSSSCG00000041959-IRAK1 might be important regulatory networks affecting Toll-like receptor signaling pathway.The result of Real-time quantitative RT-PCR was consistent with those of sequencing.【Conclusion】In this study,lncRNA-ENSSSCG00000041959-RIPK1 and lncRNA-ENSSSCG00000041959-IRAK1 were initially identified as the lncRNA-mRNA regulatory network affecting the Toll-like receptor signaling pathway,which laid a theoretical foundation for further exploration of lncRNA regulation of the immune response of ASFV infection.

Key words: African swine fever virus (ASFV); lncRNA; Toll-like receptor signaling pathway; lncRNA-mRNA regulatory network

CLC Number:

S852.65^＋1

CHEN Shiyu, CUI Shuai, JIANG Yajun, XIN Ting, WANG Yang, HAO Yuxin, HUANG Jianxin, GUO Xiaoyu, ZHU Hongfei, WU Jiajun, JIA Hong. lncRNA-mRNA Analysis of Toll-like Receptor Signaling in Porcine Peripheral Blood Lymphocytes After African Swine Fever Virus Infection[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2677-2687.

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lncRNA-mRNA Analysis of Toll-like Receptor Signaling in Porcine Peripheral Blood Lymphocytes After African Swine Fever Virus Infection

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