乏情和发情初产母猪卵巢microRNA差异表达分析

doi:10.16431/j.cnki.1671-7236.2018.05.004

Abstract

Abstract:

To explore the role of ovarian microRNA (miRNA) in regulating the reproductive process in primiparous sows,the miRNA expression profiles were examined using Illumina high-throughout sequencing.The miRNA with high expression and significant difference were analyzed by bioinformatics methods.The results showed that a total of 503 miRNAs were detected in two small RNA libraries,which included 303 known and 200 novel miRNAs.The 3 most highly expressed miRNAs in known miRNAs were ssc-miR-10b,ssc-miR-143-3p and ssc-miR-26a in two small RNA libraries.The highest specific expressed miRNAs in novel miRNAs was chr13_2637_mature,followed by chr8_9994_mature.Compared with estrous primiparous sows,a total of 145 differentially expressed miRNAs (read counts>10,|log₂(fold-change)|>1),including 114 upregulated miRNAs and 31 downregulated miRNAs were identified.31 differentially expressed miRNAs (read counts>1 000,∣log₂(fold-change)∣>1) were further screened.In these miRNAs,chr13_2585_mature was the highest-upregulated,and was discovered only in the ovary of anestrous primiparous sows.Furthermore,7 388 target genes were predicted from 31 miRNAs.KEGG pathway analyses showed that a total of 2 788 target genes were annotated to 297 pathways,and the top 20 most significantly enriched pathways were involved in reproductive processes,suggesting the 31 miRNAs played a crucial role in the reproductive process of primiparous sows.These results enriched the porcine miRNA database and provided useful information for further research about the reproductive performance of primiparous sows.

Key words: miRNAs; anestrus; estrus; primiparous sow; ovary

CLC Number:

S828

REN Qiaoling, BAI Xianxiao, WANG Jing, GUO Hongxia, CHEN Junfeng, MA Wentao, HE Xiaoe, ZHANG Jiaqing, XING Baosong. Analysis on Differentially Expressed microRNA in Ovaries of Anestrous and Estrous Primiparous Sows[J]. , 2018, 45(5): 1153-1162.

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Analysis on Differentially Expressed microRNA in Ovaries of Anestrous and Estrous Primiparous Sows

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