基于高通量测序的水貂胚胎滞育期和激活期卵巢转录组分析

doi:10.16431/j.cnki.1671-7236.2021.11.013

Abstract

Abstract: This experiment was aimed to obtain the mink ovarian transcriptome at the embryonic diapause and activation stages, dig the differentially expressed genes (DEGs) between them, and explore the biological functions of DEGs, which could provide a reference for investigating the molecular mechanisms underlying ovarian signals regulate the embryonic diapause and activation. Ovaries were collected randomly from eight healthy female mink (four mink for embryonic diapause and activation stages) were processed for transcriptomic sequencing using Illumina HiSeq platform RNA-Seq technology. Ovarian DEGs were screened and analyzed through bioinformatics. The results showed that a total of 661 195 568 raw reads were obtained by sequencing, 650 834 900 clean reads were obtained by filtering, and 389 895 unigenes were obtained by assembly. unigenes was annotated by comparing with Nr, GO, KOG and KEGG databases. 156 419, 122 657, 58 320 and 72 653 unigenes were annotated in Nr, GO, KOG and KEGG databases, respectively. There were 1 797 DEGs in mink ovaries at the embryonic diapause and activation stages. Compared with the embryonic diapause mink ovary, 1 298 DEGs in the activation stage ovaries were significantly up-regulated and 499 DEGs were significantly down-regulated. GO function analysis showed that the biological processes of DEGs significantly enriched mainly included transmembrane signaling receptor activity, signaling receptor activity, G-protein coupled receptor activity, enzyme linked receptor protein signaling pathway, cell surface receptor signaling pathway, cell cycle arrest, arylesterase activity. KEGG pathway analysis showed that the DEGs in the mink ovaries during embryonic diapause and activation stages were significantly enriched in the neuroactive ligand-receptor interaction. In this study, high-throughput sequencing technology was used to obtain transcriptome information of mink ovaries during embryonic diapause stage and during embryonic activation stage, which laid a foundation for further investigating the roles of mink ovary in regulating embryonic diapause and activation.

Key words: mink; ovary; embryonic diapause stage; embryonic activation stage; transcriptome; RNA-Seq

CLC Number:

S814.8

HAN Yuping, ZHAO Xiangyuan, FAN Bingfeng, LIU Lixiang, SHAO Jing, XU Baozeng. Transcriptomic Analysis of Mink Ovaries at Embryonic Diapause and Activation Stages Based on High-throughput Sequencing Technology[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(11): 4035-4046.

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Transcriptomic Analysis of Mink Ovaries at Embryonic Diapause and Activation Stages Based on High-throughput Sequencing Technology

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