甘草查耳酮A对貂源铜绿假单胞菌的抗菌活性

doi:10.16431/j.cnki.1671-7236.2022.03.041

Abstract

Abstract: 【Objective】 The aim of this experiment was to study the antibacterial activity of licochalcone A against Pseudomonas aeruginosa isolated from mink, and to provide experimental basis for the rational use of licochalcone A on the infective treatment clinically.【Method】 The minimal inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of licochalcone A against Pseudomonas aeruginosa were determined by microbroth dilution method and plate counting method, respectively.The germicidal efficacy of 1MIC, 2MIC, 4MIC and 8MIC of licochalcone A against Pseudomonas aeruginosa was determined by plate counting method.The effect of 1/16MIC, 1/8MIC, 1/4MIC and 1/2MIC of licochalcone A on the growth of Pseudomonas aeruginosa was determined by absorbance method.The crystal violet staining was used for the determination of the biofilm inhibition with 1/8MIC, 1/4MIC, 1/2MIC, and 1MIC of licochalcone A and the biofilm clearance with 1/4MIC, 1/2MIC, 1MIC and 1MBC of licochalcone A.The model of skin wound and skin abscess were established in mice to evaluate the therapeutic effect of licochalcone A on Pseudomonas aeruginosa infected mice.【Result】 Licochalcone A showed a good antibacterial activity against Pseudomonas aeruginosa from mink.The MIC and MBC values were 3.125 and 12.5 μg/mL, respectively.The results of time-bactericidal curve showed that the number of Pseudomonas aeruginosa was decreased gradually in 2MIC and 1MIC groups within 6 hours, and then remained at about 1×10⁵ CFU/mL, while in 8MIC and 4MIC groups the number of Pseudomonas aeruginosa was in a downward trend, and no colonies were formed after 14 and 16 hours, respectively.Licochalcone A with 1/16MIC had little effect on the growth of Pseudomonas aeruginosa, but 1/8MIC, 1/4MIC and 1/2MIC could inhibit the growth of Pseudomonas aeruginosa.The inhibitory rate of licochalcone A with 1MIC on biofilm formation of Pseudomonas aeruginosa was about 70%, and 1MBC could clear about 50% of preformed biofilm.The results of in vivo experiments showed that licochalcone A could promote wound healing and reduce the formation of skin abscess in mice infected with Pseudomonas aeruginosa.Compared with the control group, the number of bacteria in abscess in licochalcone A and ampicillin groups was extremely significantly decreased (P<0.01).【Conclusion】 Licochalcone A had good antibacterial activity against Pseudomonas aeruginosa isolated from mink in vitro and in vivo, and could be further used in the development of the preparation for clinical treatment of Pseudomonas aeruginosa infection.

Key words: licochalcone A; Pseudomonas aeruginosa; mink; antibacterial activity

CLC Number:

S852.61

GUO Jinxiang, LYU Huiru, LI Jiaofeng, LIAO Chengshui, ZHANG Linlin, NIU Junhui. Antibacterial Activity of Licochalcone A Against Pseudomonas aeruginosa Isolated from Mink[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1181-1188.

References

[1] AZAM M W, KHAN A U.Updates on the pathogenicity status of Pseudomonas aeruginosa[J].Drug Discovery Today, 2019, 24(1):350-359.
[2] 张明亮, 张淼丹, 闫新武, 等.貂源铜绿假单胞菌耐药性及生物学特性[J].河南农业科学, 2019, 48(5):122-129. ZHANG M L, ZHANG M D, YAN X W, et al.Resistance and biological characteristics of Pseudomonas aeruginosa from mink[J].Journal of Henan Agricultural Sciences, 2019, 48(5):122-129.(in Chinese)
[3] 张庆勋, 景胜凡, 韩姝伊, 等.水貂源铜绿假单胞菌的分离鉴定、耐药性及毒力基因检测[J].中国畜牧兽医, 2021, 48(6):2230-2237. ZHANG Q X, JING S F, HAN S Y, et al.Isolation, identification, drug resistance and virulence genes detection of Pseudomonas aeruginosa from mink[J].China Animal Husbandry & Veterinary Medicine, 2021, 48(6):2230-2237.(in Chinese)
[4] 郑相阔, 田学斌, 方人驰, 等.龙血竭对分离自伤口感染的铜绿假单胞菌的抗菌活性研究[J].中国抗生素杂志, 2020, 45(10):1078-1083. ZHENG X K, TIAN X B, FANG R C, et al.Antibacterial activities of dragon's blood against Pseudomonas aeruginosa isolated from the wound infections[J].Chinese Journal of Antibiotics, 2020, 45(10):1078-1083.(in Chinese)
[5] 全国细菌耐药监测网.全国细菌耐药监测网2014-2019年临床伤口及脓液标本细菌耐药监测报告[J].中国感染控制杂志, 2021, 20(2):145-156. CHINA ANTIMICROBIAL RESISTANCE SURVEILLANCE SYSTEM.Antimicrobial resistance of bacteria from wound and pus specimens:Surveillance report from China antimicrobial resistance surveillance system in 2014-2019[J].Chinese Journal of Infection Control, 2021, 20(2):145-156.(in Chinese)
[6] 孟菲, 黎书长, 杨武宁, 等.铜绿假单胞菌生物膜研究进展[J].中国畜牧兽医, 2016, 43(11):2976-2981. MENG F, LI S C, YANG W N, et al.Research progress on biofilms in Pseudomonas aeruginosa[J].China Animal Husbandry & Veterinary Medicine, 2016, 43(11):2976-2981.(in Chinese)
[7] PANG Z, RAUDONIS R, GLICK B R, et al.Antibiotic resistance in Pseudomonas aeruginosa:Mechanisms and alternative therapeutic strategies[J].Biotechnology Advances, 2019, 37(1):177-192.
[8] HASAN M K, ARA I, MONDAL M S A, et al.Phytochemistry, pharmacological activity, and potential health benefits of Glycyrrhiza glabra[J].Heliyon, 2021, 7(6):e07240.
[9] CHAKOTIYA A S, TANWAR A, SRIVASTAVA P, et al.Effect of aquo-alchoholic extract of Glycyrrhiza glabra against Pseudomonas aeruginosa in mice lung infection model[J].Biomedicine & Pharmacotherapy, 2017, 90:171-178.
[10] WANG Z F, LIU J, YANG Y A, et al.A review:The anti-inflammatory, anticancer and antibacterial properties of four kinds of licorice flavonoids isolated from licorice[J].Current Medicinal Chemistry, 2020, 27(12):1997-2011.
[11] 鲍佳佳, 李倩, 孙铭艳, 等.ε-聚赖氨酸对铜绿假单胞菌生长及生物膜形成的影响[J].中国病原生物学杂质, 2021, 16(3):258-265. BAO J J, LI Q, SUN M Y, et al.The effect of ε-polylysine on the growth of and biofilm formation by Pseudomonas aeruginosa[J].Journal of Pathogen Biology, 2021, 16(3):258-265.(in Chinese)
[12] 廖成水, 贾艳艳, 李智丽, 等.甘草多糖对单核细胞增生性李斯特菌体内外生长的影响[J].食品科学, 2016, 37(17):213-218. LIAO C S, JIA Y Y, LI Z L, et al.Effect of Glycyrrhiza polysaccharide on the growth of Listeria monocytogenes in vitro and in vivo[J].Food Science, 2016, 37(17):213-218.(in Chinese)
[13] SAMAD A, KHAN A A, SAJID M, et al.Assessment of biofilm formation by Pseudomonas aeruginosa and hydrodynamic evaluation of microtiter plate assay[J].Journal of the Pakistan Medical Association, 2019, 69(5):666-671.
[14] HONG W, GAO X, QIU P, et al.Synthesis, construction, and evaluation of self-assembled nano-bacitracin A as an efficient antibacterial agent in vitro and in vivo[J].International Journal of Nanomedicine, 2017, 12:4691-4708.
[15] ALFORD M A, BAQUIR B, AN A, et al.NtrBC selectively regulates host-pathogen interactions, virulence, and ciprofloxacin susceptibility of Pseudomonas aeruginosa[J].Frontiers in Cellular and Infection Microbiology, 2021, 11:694789.
[16] 杨静, 常小强, 王霞, 等.甘草活性成分提取及抑菌活性研究[J].河南科学, 2017, 35(10):1587-1591. YANG J, CHANG X Q, WANG X, et al.Extraction and antimicrobal activity of active components from glycyrrhiza[J].Henan Science, 2017, 35(10):1587-1591.(in Chinese)
[17] 申凤鸽.甘草查耳酮A抗金黄色葡萄球菌生物被膜的分子机制初步研究[D].长春:吉林大学, 2013. SHEN F G.The preliminary study on the molecular mechanism of licochalcone A against Staphylococcus aureus biofilm[D].Changchun:Jilin University, 2013.(in Chinese)
[18] 曾爱英, 廖星, 戴木森.磷霉素联合美罗培南对铜绿假单胞菌生物被膜的影响[J].实用中西医结合临床, 2021, 21(12):158-159. ZENG A Y, LIAO X, DAI M S.Effects of fosfomycin combined with meropenem on the biofilm of Pseudomonas aeruginosa[J].Practical Clinical Journal of Integrated Traditional Chinese and Western Medicine, 2021, 21(12):158-159.(in Chinese)

Antibacterial Activity of Licochalcone A Against Pseudomonas aeruginosa Isolated from Mink

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