牛至油博落回口服液的研制及体外抑菌活性测定

doi:10.16431/j.cnki.1671-7236.2022.02.034

Abstract

Abstract: 【Objective】 This study was aimed to prepare oregano oil Macleaya cordata oral solution, determine its antibacterial activity in vitro and provide theoretical reference for clinical use of new veterinary drugs.【Method】 The formulae were optimized by pre-test and Box-Behnken response surface method.The main components of oral solutiom were determined by high performance liquid chromatography (HPLC).The inhibition zone diameters of oral solutiona against Escherichia coli, Salmonella, Staphylococcus aureus and Streptococcus faecalis were determined by disk diffusion method.The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of oral solution, 5% oregano oil solution and 1% Macleaya cordata solution against 4 kinds of bacteria were determined by tube double dilution method.The combined susceptibility test in vitro of 5% oregano oil solution and 1% Macleaya cordata solution was carried out with checkerboard microdilution test.【Result】 The optimal formula of oregano oil Macleaya cordata oral solution was 5% oregano oil, 1% Macleaya cordata, 25% solubilizer RH40, 0.02% antioxidant BHT and water.The concentration of carvacrol and sanguinarine was 42.59 and 6.51 mg/mL in oral solution, respectively.The inhibition zone diameters of oral solution to 4 bacteria were 16.9, 16.4, 23.7 and 17.0 mm, MIC were 3.125, 3.125, 1.5625 and 1.5625 μL/mL, and MBC were 12.5, 6.25, 3.125 and 3.125 μL/mL, respectively; The MIC of 5% oregano oil solution to 4 bacteria were 25, 12.5, 6.25 and 100 μL/mL, and MBC were 100, 50, 25 and > 200 μL/mL, respectively.The MIC of 1% Macleaya cordata solution to 4 bacteria were 6.25, 12.5, 3.125 and 6.25 μL/mL, and MBC were 50, 25, 6.25 and 25 μL/mL, respectively.The combined susceptibility test showed that the two combined components had additive effect on Escherichia coli and Salmonella, irrelevant effect on Staphylococcus aureus, and synergistic effect on Streptococcus faecalis.【Conclusion】 Oregano oil Macleaya cordata oral solution was prepared in this study, it had good inhibitory effects on Escherichia coli, Salmonella, Staphylococcus aureus and Streptococcus faecalis.

Key words: oregano oil; Macleaya cordata; response surface method; antibacterial activity

CLC Number:

S852.61

LI Weihao, SUN Yuanyuan, LIU Juxiang. Preparation of Oregano Oil Macleaya cordata Oral Solution and Its Antibacterial Activity in vitro[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 718-730.

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Preparation of Oregano Oil Macleaya cordata Oral Solution and Its Antibacterial Activity in vitro

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