杂交构树叶多酚提取工艺优化及其抗氧化活性研究

doi:10.16431/j.cnki.1671-7236.2021.03.029

Abstract

Abstract: In order to establish and optimize the extraction process of polyphenols from Broussonetia papyrifera leaves,the ultrasonic assisted extraction method was used in this experiment.Taking the yield of polyphenols from Broussonetia papyrifera leaves as the index,the effects of the material to solvent ratio,ethanol concentration,ultrasonic time and ultrasonic temperature on the yield of polyphenols from Broussonetia papyrifera leaves were studied by single factor experiment.On the basis of single factor experiment,the experiment of 4 factors and 3 levels was designed by response surface methodology(RSM) to optimize the extraction process parameters of Broussonetia papyrifera leaf polyphenols,a regression model was established and response surface analysis was carried out to determine the optimal extraction process parameters,and three parallel validation were carried out.Taking vitamin C as the control,the antioxidant activity of Broussonetia papyrifera leaf polyphenols on DPPH and ABTS free radical scavenging rate was determined to evaluate in vitro antioxidant activity of Broussonetia papyrifera leaf polyphenols.The results showed that the order of the four factors on polyphenol yield was ethanol concentration (B)>the material to solvent ratio (A)>ultrasonic temperature(D)>ultrasonic time(C).RSM results showed that there was interaction between the two factors,but the interaction was not significant (P>0.05).When the material to solvent ratio was 70:1,ethanol concentration was 60%,ultrasonic time was 50 min,and ultrasonic temperature was 50 ℃,the yield of polyphenols from Broussonetia papyrifera leaves was 13.62 mg/g,which was close to the predicted value of RSM.In vitro antioxidant test showed that Broussonetia papyrifera leaf polyphenols could scavenge DPPH and ABTS free radicals,and could reach the level equivalent to vitamin C at high concentration.In conclusion,the extraction process of polyphenols from Broussonetia papyrifera leaves in this study was accurate and feasible.It was also proved that the polyphenols from Broussonetia papyrifera leaves had good antioxidant activity.The results could provide reference for the study of biological activity of Broussonetia papyrifera leaves and the development of medicinal value of Broussonetia papyrifera leaves.

Key words: Broussonetia papyrifera leaves; polyphenols; ultrasonic; response surface methodology; antioxidant activity

CLC Number:

S853.7

XU Baichang, FANG Ziyi, MENG Jinyan, CUI Qinghan, LI Hongman, SI Hongbin. Optimization of Extraction Process and Antioxidant Activity of Polyphenols from Leaves of Broussonetia papyrifera[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(3): 1046-1053.

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Optimization of Extraction Process and Antioxidant Activity of Polyphenols from Leaves of Broussonetia papyrifera

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