蛹虫草多糖对注射环磷酰胺小鼠抗氧化功能的影响

doi:10.16431/j.cnki.1671-7236.2016.12.025

Abstract

Abstract:

To investigate the antioxidant effects of the Cordyceps militaris polysaccharides(CMP),90 male BALB/c mice were randomly divided into six groups, three experimental groups (CMP groups), model control group (CY group), blank control group (BC group) and positive control group (PC group). The mice in CMP and CY groups were given cyclophosphamide at 80 mg/kg via intraperitoneal injection for 3 d. Then the CMP groups were administrated 17.5, 35.0 and 70.0 mg/(kg·BW) CMP via gavage, respectively, BC and CY groups were given physiological saline, and PC group was orally administered 70 mg/(kg·BW) CMP, lasting 18 d. At 24 h after the last administration, heart, liver and kidney were collected to measure the levels of MAD, CAT, SOD, GSH-Px and T-AOC in the homogenate.SOD and TAOC activity of three CMP groups increased significantly (P<0.01), and the level of MDA decreased significantly (P<0.01) compared with CY group. As for CAT activity of heart, the value in low-dose CMP group had no significant change (P>0.05), while in middle and high-dose groups had significant (P<0.05) and extremely significant(P<0.01) increase respectively. Except that the hepatic GSH-Px activity increased significantly (P<0.05) in low-dose CMP group, the rest values of GSH-Px activities in all CMP groups increased extremely significantly (P<0.01). The results indicated that CMP could effectively improve the antioxidant function of mice and had the potential to be a good resource of new antioxidant drugs.

Key words: Cordyceps militaris polysaccharides; cyclophosphamide; antioxidant activity; mice

CLC Number:

S853.7

WANG Mi, WANG Xiao-yang, ZHANG Li-fang, FEI Chen-zhong, ZHANG Ke-yu, WANG Chun-mei, YANG Rui-le, XUE Fei-qun. The Effect of Cordyceps Militaris Polysaccharides on the Antioxidant Function in Mice Injected With Cyclophosphamide[J]. , 2016, 43(12): 3251-3256.

References

[1] Sun X, Sun Y, Zhang Q, et al. Screening and comparison of antioxidant activities of polysaccharides from Coriolus versicolor[J]. International Journal of Biological Macromolecules, 2014, 69:12-19.
[2] 凌关庭. 氧化·疾病·抗氧化(Ⅳ)[J].粮食与油脂, 2003, 12:49-51.
[3] Li S Q, Shah N P. Antioxidant and antibacterial activities of sulphated polysaccharides from Pleurotus eryngii and Streptococcus thermophilus ASCC 1275[J]. Food Chemistry, 2014, 165(3):262-270.
[4] Qi H M, Zhang Q B, Zhao T T, et al. In vitro antioxidant activity of acetylated and benzoylated derivatives of polysaccharide extracted from Ulva pertusa (Chlorophyta)[J]. Bioorganic & Medicinal Chemistry Letters, 2006, 16(9):2441-2445.
[5] Wu G H, Hu T, Li Z Y, et al. In vitro antioxidant activities of the polysaccharides from Pleurotus tuber-regium (Fr.) Sing[J]. Food Chemistry, 2014, 148(4):351-356.
[6] 陈芬芳. 刺五加多糖对肉鸡免疫功能和抗氧化性能的研究[D]. 聊城:聊城大学, 2015.
[7] Lin H Y, Chou C C. Antioxidative activities of water soluble disaccharide chitosan derivatives[J].Food Research International, 2004, 37(9):883-889.
[8] Yasser F M K, Hanan M A A. Free-radical scavenging and antioxidative activities of some polysaccharides in emulsions[J].Food Science and Technology, 2007, 40(2):270-277.
[9] Kagimura F Y, da Cunha M A, Theis T V, et al. Carboxymethylation of (1→6)-β-glucan (lasiodiplodan):Preparation, characterization and antioxidant evaluation[J]. Carbohydrate Polymers, 2015, 127:390-399.
[10] 王珅,毕聪明,陈强,等. 蒲公英多糖对肉鸡血清生化指标及抗氧化指标的影响[J]. 畜牧与兽医, 2015,47(5):74-75.
[11] 卢炜,顾蓓蓓,赵莎莎.黄芪多糖对幼犬淋巴细胞转化的影响及其抗氧化作用初探[J].中国畜牧兽医文摘,2015,31(11):225-226.
[12] 张彩利,刘玉姣,王安忠,等. 枸杞多糖对己烯雌酚生殖损伤仓鼠抗氧化能力及生殖激素水平的影响[J]. 中国兽医学报,2014,34(8):1364-1368.
[13] Blaszczyk K, Wilczak J, Harasym J, et al. Impact of low and high molecular weight oat beta-glucan on oxidative stress and antioxidant defense in spleen of rats with LPS induced enteritis[J]. Food Hydrocolloids, 2015,51:272-280.
[14] Yu R M, Yang W, Song L Y, et al. Structural characterization and antioxidant activity of a polysaccharide from the fruiting bodies of cultured Cordyceps militaris[J]. Carbohydrate Polymers, 2007, 70(4):430-436.
[15] Yu R M, Yin Y, Yang W, et al. Structural elucidation and biological activity of a novel polysaccharideby alkaline extraction from cultured Cordyceps militaris[J]. Carbohydrate Polymers, 2009, 75(1):166-171.
[16] 代卉,乐国伟,孙进,等. 小麦肽对受环磷酰胺免疫抑制小鼠的免疫调节及抗氧化功能[J]. 生物工程学报, 2009, 25(4):549-555.
[17] 杨义芳,孔德云.中药提取分离手册[M].北京:化学工业出版社,2009.
[18] Simic M G, Bergtold D S, Karam L R, et al. Generation of oxygen radicals in biosystems[J]. Mutation Research, 1989, 214(1):3-12.
[19] Zheng R L, Huang Z Y. Reactive oxygen species[A]. Free Radical in Medical and Agricultural Science[C]. 2001.
[20] Liu F, Ooi V E, Chang S T. Free radical scavenging activities of mushroom polysaccharide extracts[J]. Life Science,1997, 60(10):763-771.
[21] Peterszegi G, Robert A M, Robert L. Protection by L-fucose and fucose-rich polysaccharides against ROS-produced cell death in presence of ascorbate free radical scavenging activities of mushroom polysaccharide extracts[J]. Biomed Pharmacother, 2003, 57(3-4):130-133.
[22] Zhang Q B, Li N, Zhou G F, et al. In vivo antioxidant activity of polysaccharide fraction from Porphyra haitanesis (Rhodephyta) in aging mice[J]. Pharmacology Research, 2003, 48(2):151-155.
[23] 张海玉,朴惠顺,金在久,等. 参芪合剂对环磷酰胺所致免疫抑制小鼠免疫和抗氧化功能的影响[J]. 吉林大学学报,2009,35(5):857-861.
[24] Chen C, Luo S S, Li Y, et al.Study on antioxidant activity of three Cordyceps sp. by chemiluminescence[J]. Shanghai Journal of Traditional Chinese Medicine, 2007, 38(7):53-55.

The Effect of Cordyceps Militaris Polysaccharides on the Antioxidant Function in Mice Injected With Cyclophosphamide

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	ZHANG Xiaomeng, JIAO Anhui, WANG Yuqi, FAN Nanzhu, JIN Qingguo. Effects of Rosiglitazone Supplementation on Redox Homeostasis of Oocytes in Mice [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1818-1827.
[2]	LU Hui, CAI Gaofeng, WU Caihong, QIU Shulei, YUAN Chen, CHEN Xiaolan, LIU Yun, QIN Kaili. Effect of Agrocybe aegerita Polysaccharid on Immunomodulatory of Splenic Lymphocytes in Mice [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1828-1835.
[3]	SHAO Yongbin, ZHENG Yichuang, ZHANG Jingyi, WEI Linying, TIAN Yong, SUN Guojie, LIU Yuran, LUO Yan, GU Xinli. Study on the Anti-inflammatory Effect of Leonurine on LPS-induced Endometritis in Mice [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2149-2155.
[4]	SHI Hongzhao, LIU Yang, YAO Ruizhi, LIU Peng, LI Shize. Effects of Cold Stress Rewarming on Neurobehavioral Changes in ICR Mice [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 932-941.
[5]	XU Qianqian, YANG Wanqun, WANG Yubo, WU Fei, DAI Juanjuan, QIN Mimi, ZHUANG Jinqiu, WANG Yan, SHEN Zhiqiang, LI Jichang. Study on Immunomodulatory Effect of Prepared Radix Rehmanniae Polysaccharide on Cyclophosphamide-induced Immunosuppression in Mice [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 693-703.
[6]	WANG Wei, LI Chunzhu, WANG Shasha, SANG Rui, GE Bingjie, LI Chunting, ZHAO Xin, LIU Xinman, YU Minghong, ZHANG Xuemei. Study on the Protective Mechanism of Traditional Chinese Medicine Combined with Probiotics on Diarrhea Caused by E.coli in Mice [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 779-788.
[7]	LI Jiahao, LI Chao, ZANG Changjiang, MENG Jun, LI Hongkun, JIA Yiqi, MAILIKEZHATI Aierken, TANGNAER Tuoliubieke. Effects of Gastric and Intraperitoneal Injection of Lactobacillus rhamnosus on Fecal Flora Diversity in Mice During Pregnancy [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 151-160.
[8]	YU Kai, ZHAO Yufen, WENG Yu, WANG Qin, HAO Shaoyu, YU Boyang, DU Chenguang, SUBUDENG Gerile, LI Haijun. Effects of Dihydrotestosterone on Proliferation and Expression of Anti-Müllerian Hormone of Mouse Granulosa Cells [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3036-3043.
[9]	LI Chunting, WANG Shasha, ZHAO Xin, WANG Wei, GE Bingjie, ZHANG Xuemei. Protective Effect of Traditional Chinese Medicine Combined with Probiotics on E.coli Diarrheain Mice [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3226-3234.
[10]	PAN Jianqiu, LIUFU Sui, WU Manli, JIANG Danli, OUYANG Hongjia, SHEN Xu, XU Danning, HE Jianhua, HUANG Yunmao. Effects of Exogenous MLT and RFRP-3 on Growth and Reproductive Performance of Male Mice at the Puberty [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2622-2630.
[11]	TIAN Wei, FANG Yanya, LIU Haixing, LI Zhongshu. Effect of bFGF on in vitro Development of Mouse Zygotes Treated with 3-nitropropionic Acid [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 608-614.
[12]	YIN Huihui, TONG Yanmei, ZENG Xueyan, LU Guangqiang, ZHAO Wu, JIANG Yuanming, LIU Wei. Effect of Polysaccharide from Rhodomyrtus tomentosa Fruit on Immunological Function in Immunosuppressed Mice [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 731-737.
[13]	ZHANG Yuanyuan, TIAN Zhichen, SUN Yujia, ZHANG Huimin, YANG Zhangping, WANG Cong, CAO Ke, MAO Yongjiang, LI Mingxun. Effects of Taurine Supplementation on Glycolipid Metabolism in C57BL/6J Mice with High-fat Diet [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(12): 4604-4613.
[14]	GUO Zimeng, WANG Fuzheng, WANG Chengzhi, DU Peng, ZHANG Mingyue, LI Zeyao, SU Xiaochen, WU Gaofeng, YANG Jiancheng. Effect of Taurine Additive Package on the Immune Function of Immunosuppressed Mice [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(12): 4635-4645.
[15]	ZHAO Weijie, SUN Yaogui, SUN Panpan, SUN Na, YANG Huizhen, LI Hongquan. Study on Repair Effect and Mechanism of Nigella sativa Seed Extract on Alcoholic Liver Injury in Mice [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(12): 4928-4935.