一株高抗氧化活性戊糖片球菌的筛选

doi:10.16431/j.cnki.1671-7236.2022.01.019

Abstract

Abstract: [Objective] In this experiment, lactic acid bacteria with high antioxidant activity were screened and identified in order to provide a new antioxidant microecological agent for alleviating oxidative stress in the process of livestock and poultry breeding. [Method] Lactic acid bacteria with significant antioxidant capacity were screened from 27 strains, by establishing a primary screening method for tolerance to hydrogen peroxide oxidative stress, and taking the tolerance survival rate to hydrogen peroxide as the re-screening index. Then the antioxidant activity of lactic acid bacteria was evaluated by DPPH free radical (DPPH·) and hydroxyl free radical (HO·) scavenging ability, reducing activity, anti lipid peroxidation ability, total superoxide dismutase (T-SOD) and total antioxidant capacity (T-AOC) in vitro. [Result] A Lactobacillus C2-0327 with excellent antioxidant activity was screened from the 27 strains. Scavenging rates of DPPH· and HO· of this isolate C2-0327 were 95.2% and 141.0%, respectively. The reducing activities of this isolate reached 3 147 μmol/L (L-cysteine equivalent), and inhibition rate of lipid peroxidation was up to 82.9%. T-SOD activity and T-AOC were 81.32 and 20.10 U/mL, respectively. The strain was identified as Pediococcus pentosus C2-0327 by 16S rDNA molecular identification. [Conclusion] A strain of Lactobacillus with high antioxidant activity was screened and identified, which was Pediococcus pentosus C2-0327. The high antioxidant Pediococcus pentosus could be used for the development of new antioxidant probiotics.

Key words: lactic acid bacteria; antioxidant activity; screening; Pediococcus pentosu

CLC Number:

S816.7

WANG Xiangyan, XIN Guoqin, XU Haiyan, GU Wei, LAN Jianghua, HAO Muqiang, FENG Gang. Screening of a Strain of Pediococcus pentosus with High Antioxidant Activity[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(1): 179-187.

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Screening of a Strain of Pediococcus pentosus with High Antioxidant Activity

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