甘肃棘豆黄酮抗氧化活性及免疫活性的研究

doi:10.16431/j.cnki.1671-7236.2018.12.010

Abstract

Abstract:

To investigate the antioxidation and immune activity of Oxytropis kansuensis flavonoids,the antioxidant activities of Oxytropis kansuensis flavonoids were determined by DPPH,superoxide anion and hydroxyl free radical scavenging activities,and the immunomodulatory of Oxytropis kansuensis flavonoids were studied by measuring the effects of different concentrations of Oxytropis kansuensis flavonoids on the proliferation of mouse spleen lymphocytes,the concentrations of IL-2 and IgG.The results showed that the highest scavenging rate of DPPH free radicals of Oxytropis kansuensis flavonoids was 32.23% which was slightly higher than vitamin E (28.55%) and lower than vitamin C (97.05%).The highest scavenging rate of superoxide anion free radicals of Oxytropis kansuensis flavonoids was 80.03% which was higher than vitamin E (69.70%) and lower than vitamin C (98.69%).The highest clearance rate of hydroxyl free radicals of Oxytropis kansuensis flavonoids was 30.39% which was lower than that of vitamin C (98.98%) and slightly lower than that of vitamin E (32.57%).In the spleen lymphocyte proliferation assay on mice,the relative proliferation rate of spleen lymphocytes increased with the increase concentration of Oxytropis kansuensis flavonoids.When the concentration of Oxytropis kansuensis flavonoids was 200 μg/mL,the spleen lymphocyte proliferation rate was 23.40%.The IL-2 concentration in 200 μg/mL Oxytropis kansuensis flavonoids group was extremely significantly higher than that of control group (P<0.01),and there was no significant difference between 50,100 μg/mL Oxytropis kansuensis flavonoids groups and control group (P>0.05);The IgG antibody concentration of Oxytropis kansuensis flavonoids groups were extremely significantly higher than that of control group (P<0.01),50 μg/mL Oxytropis kansuensis flavonoids group was extremely significantly higher than that of 100 and 200 μg/mL Oxytropis kansuensis flavonoids groups (P<0.01),and 100 μg/mL Oxytropis kansuensis flavonoids group was extremely significantly higher than 200 μg/mL Oxytropis kansuensis flavonoids group (P<0.01).In conclusion,the Oxytropis kansuensis flavonoids had good antioxidant and immune activity.

Key words: Oxytropis kansuensis flavonoids; antioxidant activity; immune activity; lymphocyte proliferation; IgG antibody

CLC Number:

S859.3

ZHANG Fuxin, SONG Jiaxuan, LIU Xiaodong. Antioxidation and Immune Activity of Oxytropis kansuensis flavonoids[J]. , 2018, 45(12): 3387-3394.

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Antioxidation and Immune Activity of Oxytropis kansuensis flavonoids

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