红茂草中精油成分分析及抑菌活性的研究

doi:10.16431/j.cnki.1671-7236.2020.05.036

Abstract

Abstract: For the further development and utilization of the drug,wild medicinal plant Dicranostigma leptopodum (Maxim.) Fedde in Pingliang area of Gansu province was sekcted in this experiment as the research object,the essential oil components were extracted by steam extraction,distillation extraction (SDE) and supercritical CO₂ extraction.Chemical composition of essential oil was analyzed by GC-MS,and the antibacterial activity of supercritical CO₂ extracted essential oil was determined against Escherichia coli,Staphylococcus aureus and Pseudomonas aeruginosa using plate count method.The results showed that the essential oil obtained by steam extraction and distillation extraction was orange yellow and turbid.The essential oil obtained by supercritical CO₂ extraction was light yellow and clear liquid.The essential oil extracted by the 3 methods was all contained 24 common components.The supercritical CO₂ extraction method was the best,and the extraction rate was 2.1220%.The steam extraction method was the lowest,and the extraction rate was 1.7870%.Supercritical CO₂ extracted essential oil was applied to 3 strains of bacteria tested,and the minimal inhibitory concentration (MIC) was determined as Escherichia coli 0.2500 g/mL,Staphylococcus aureus 0.3000 g/mL and Pseudomonas aeruginosa 0.2600 g/mL,respectively.The bactericidal inhibitory effect was significant.The results showed that supercritical CO₂ extraction was the best method to extract the essential oil of Dicranostigma leptopodum (Maxim.) Fedde,which had good antibacterial effect on Escherichia coli,Staphylococcus aureus and Pseudomonas aeruginosa.

Key words: supercritical CO₂ extraction; Dicranostigma leptopodum (Maxim.) Fedde; essential oil; antibacterial activity

CLC Number:

S853.7

XIE Zongxiu, LI Shangwei, ZHOU Deshun, SONG Xiangdong, JIA Ning, LIANG Jianping. Study on Essential Oil Composition Analysis and Antibacterial Activity from Dicranostigma leptopodum (Maxim.) Fedde[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(5): 1611-1617.

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Study on Essential Oil Composition Analysis and Antibacterial Activity from Dicranostigma leptopodum (Maxim.) Fedde

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