China Animal Husbandry and Veterinary Medicine ›› 2021, Vol. 48 ›› Issue (2): 755-762.doi: 10.16431/j.cnki.1671-7236.2021.02.038

• Basic Veterinary Medicine • Previous Articles     Next Articles

Effects of 12C6+ Ion Beam Irradiation on the Quality of Tea Tree Essential Oil and Its Efficacy Against Foot-and-mouth Disease Virus

LIANG Yan1,2, TAO Lei4, SONG Xiangdong3, LIU Yu3, ZHANG Fengwu4, LI Xuehu2, XING Zhijun2, LU Xihong2, LIANG Jianping2,3, FENG Jianqiang3, ZHOU Xiang2, WANG Zhen1   

  1. 1. School of Pharmacy, Lanzhou University, Lanzhou 730000, China;
    2. Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China;
    3. Lanzhou Institute of Husbandry and Pharmaceutical Sciences of CAAS, Lanzhou 730050, China;
    4. Lanzhou Vocational Technical College, Lanzhou 730070, China
  • Received:2020-05-07 Online:2021-02-20 Published:2021-02-23

Abstract: The purpose of this study was to explore the changes of the active components of tea tree essential oil after 12C6+ ion injection and evaluate the killing effect of Foot-and-mouth disease virus (FMDV).Tea tree essential oil was irradiated by 12C6+ ion beam of 50,100,150 and 200 Gy,and the changes of main components and mass deposition effects of tea tree essential oil before and after irradiation were analyzed by gas chromatography-mass spectrometer (GC-MS) and infrared chromatography.The contents of terpinene-4-ol and 1,8-eucalyptin in tea tree essential oil were detected.The safety of tea tree essential oil before and after irradiation and the essential oil diluted by 1:2 and 1:4 irradiation were injected into suchling mice to detect the clinical use of tea tree essential oil. The irradiated tea tree essential oil diluted into 1:2, 1:4, 1:6 and 1:8 was thoroughly mixed with the same amount of O-FMDV at 1:500, respectively. The mice aged 4-5 d were subcutaneously injected and observed continuously for 7 d to observe whether the mice were infected with O-FMDV.The results showed that after irradiated tea tree essential oil with 100 Gy 12C6+ ion beam,the content of pinole-4 alcohol increased from 37.90% to 39.03%,the content of 1,8-eucalyptus had no significant change,and the content of hydroxyl compounds significantly increased after irradiated tea tree essential oil.The optimal irradiation dose was 100 Gy,and the tea tree essential oil with radiation dose of 100 Gy (the maximum dilution was 1:4) had the best anti-FMDV effect in suckling mouse.Irradiation of tea tree essential oil with a dose of 100 Gy could improve the killing effect of FMDV.

Key words: 12C6+ ion beam; tea tree essential oil; mass deposition effect; Foot-and-mouth disease virus (FMDV)

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