水貂源铜绿假单胞菌的分离鉴定、耐药性及毒力基因检测

doi:10.16431/j.cnki.1671-7236.2021.06.037

Abstract

Abstract: To find out the cause of mink death in Hebei,pathogen identification was conducted by histopathological examination,bacterial isolation and culture,morphological observation,biochemical identification,drug susceptibility test,drug resistance gene and virulence gene detection and animal regression experiment.The results showed that 3 strains of Pseudomonas aeruginosa were isolated,3 strains had 100% similarity with strains of Pseudomonas aeruginosa in GenBank,and all 3 strains could cause the death of the rats.Drug susceptibility test results showed that the isolates were highly susceptible to levofloxacin,gentamycin,amikacin,polymyxin B and ceftriaxone,and were resistant to extended-spectrum β-lactamases (ESBLs),aminoglycosides,macrolides,quinolones and tetracyclines.The isolates carried ESBLs genes (bla_CTX-M1,bla_OXA-2,bla_OXA-10),carbapenemases (bla_VIM-1,bla_SPM-1,bla_KPC-1),and 17 virulence-related markers including adhesion,T1SS-T3SS,oxidativestress,quorum sensing and regulation,and phospholipids.The results would provide references for disease control,therapeutical guidance and drug administration in mink.

Key words: mink; Pseudomonas aeruginosa; isolation and identification; drug resistance

CLC Number:

R378.99⁺1

ZHANG Qingxun, JING Shengfan, HAN Shuyi, WANG Ruoqi, FENG Shengyong, HE Hongxuan. Isolation,Identification,Drug Resistance and Virulence Genes Detection of Pseudomonas aeruginosa from Mink[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(6): 2230-2237.

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Isolation,Identification,Drug Resistance and Virulence Genes Detection of Pseudomonas aeruginosa from Mink

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