水貂肺炎克雷伯菌的分离鉴定及耐药性分析

doi:10.16431/j.cnki.1671-7236.2022.02.032

Abstract

Abstract: 【Objective】 Klebsiella pneumoniae is a zoonotic opportunistic pathogen, and also one of the common pathogenic bacteria causing mink pneumonia.In order to guide rational drug use and provide the basis for treatment to 6 dead minks suffering from pneumonia provided by mink farms in Jilin province, the bacterial isolation and identification, drug resistance spectrum and drug resistance gene analysis were carried out.【Method】 Strains were isolated from samples by isolation and purification method, and identified by PCR method, and ST typing was performed by multilocus sequence typing technique(MLST) method.The pathogenicity of the strain was learned by infecting mice.Serotype and the virulence gene was detected by PCR.The susceptibility of the strains to 18 drugs was detected by agar dilution method, and the carrying status of drug-resistant genes was detected by PCR.【Result】 The results showed that 6 isolates were identified as Klebsiella pneumoniae by PCR.MLST analysis results showed that 6 strains had 6 ST types, namely ST2594, ST1782, ST2844, ST2330, ST86 and ST661, respectively.Pathogenicity analysis results showed that all 6 strains could cause the death of mice, and a total of 6 virulence genes were detected in the isolates.No more virulent serotypes were detected.The results of drug sensitivity test showed that 6 strains of Klebsiella pneumoniae were more sensitive to ceftazidime, ceftriaxone, imipenem, meropenemand amikacin, and more resistant to streptomycin, gentamicin, kanamycin, penicillin, ampicillin, ciprofloxacin, enrofloxacin, levofloxacin, doxycycline and florfenicol.There were 18 resistance genes detected in 6 strains, including bla_SHV, bla_TEM-1, bla_CTX-M-1G, aadA1, aac(3')-Ⅳ, aac(3')-Ⅱc, aph(4')-Ⅰa, aph(3')-Ⅶ, aph(3')-Ⅳ, aph(2')-Ⅰb, rmtB, qnrB, qnrD, qnrS, qepA, oqxAB, floR and mcr-1.【Conclusion】 6 isolates of Klebsiella pneumoniae had different phylogenetic evolutionand strong pathogenicity and drug resistance, they brought difficulties to clinical drug treatment.

Key words: mink; Klebsiella pneumoniae; drug sensitivity; resistance gene

CLC Number:

S852.61⁺2

CHEN Qiang, CHENG Yuening, FENG Qiuju, GUO Li, ZHANG Shuqin, TAN Bin, YI Li, ZHAO Quan, CHENG Shipeng, SUN Na. Isolation, Identification and Drug Resistance Analysis of Klebsiella pneumoniae from Minks[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 700-708.

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Isolation, Identification and Drug Resistance Analysis of Klebsiella pneumoniae from Minks

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