恩诺沙星、喹烯酮、土霉素和硫酸多黏菌素B对细胞的联合毒性研究

doi:10.16431/j.cnki.1671-7236.2022.02.031

Abstract

Abstract: 【Objective】 The study was aimed to investigate the single and combined toxic effects of enrofloxacin (ENR), quinolone (QCT), oxytetracycline (OTC) and polymyxins B sulfate (PMB) on cells.【Method】 L02, AML12, Marc145, HEK293T, TM3 and SK-N-SH cells were used as models.The inhibition rates of four drugs on six kinds of cells at different concentrations were obtained by crystal violet staining and the concentration-effect equations were fitted.EC_F(the drug concentration at F% inhibition rate (F=10, 20, 25, 33, 50)) was calculated.Binary, ternary and quaternary medicines were mixed respectively at the toxicity ratio of EC₅₀∶EC₅₀, EC₃₃∶EC₃₃∶EC₃₃, EC₂₅∶EC₂₅∶EC₂₅∶EC₂₅ and then diluted with a dilution ratio of 1/2.The inhibition rates were calculated and the actual action curves were fitted.Concentration addition model (CA) and independent action model (IA) were used to evaluate the combined effects of different drugs.【Result】 There was a dose-effect relationship between the four drugs on the growth inhibition of six kinds of cells.QCT showed the strongest toxicity while PMB showed the weakest toxicity.Binary combination showed high probability of synergism, especially the combination with OTC, and OTC+QCT showed significant synergistic inhibition in the six kinds of cells.The joint effects were prone to addition on TM3 while had a greater possibility of synergy on liver and kidney cells, and the same combination might have different effects on different cells.In ternary and quaternary combinations, the probability of additive effect increased, and the change of the combined effect was more complex, which was easy to appear multi-segment effect with the change of concentration.【Conclusion】 Common veterinary drugs had certain toxicity to liver, kidney, nerve and germ cells, and the combined toxic effects of these drugs were not simply additive, which were affected by components, concentration and so on, so incompatibility should be paid attention in the application.

Key words: veterinary medicine; joint effect; concentration addition model (CA); independent action model (IA)

CLC Number:

S859.79⁺6

FU Yuhan, HOU Lirui, ZHAO Chong, HU Hongbo, YIN Shutao. Study on Joint Toxicity of Enrofloxacin, Quinocetone, Oxytetracycline and Polymyxin B Sulfate to Cells[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 687-699.

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Study on Joint Toxicity of Enrofloxacin, Quinocetone, Oxytetracycline and Polymyxin B Sulfate to Cells

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