基于RNA-Seq测序的高、低产双黄蛋高邮鸭卵巢组织SNP筛选分析

doi:10.16431/j.cnki.1671-7236.2019.12.005

Abstract

Abstract: This study was aimed to improve the gene structure of Gaoyou duck,and explore its regulation mechanism of double-yolk egg performance.RNA-Seq technology was used to analyze the transcriptome of ovaries in high and low double-yolk egg yield Gaoyou ducks.SNP related to the double-yolk egg performance were screened by quality control and gene annotations from RNA-Seq data.Raw reads from RNA-Seq data were selected with quality control,84 540 140-87 479 660 clean reads were screened from total 6 samples,over 70.79% of the sequencing data were covered by the annotated genome data of ducks.Based on these data,108 260-116 478 SNP were found in the exon area of the annotched genes,and the number of transition SNP was extremely significant higher than the number of transversion(P<0.01).KEGG database was used to analyze the related pathways from these selected SNP.Top 20 pathways were screened,including Ribosome signaling pathway,Carbon metabolism signaling pathway,Biosynthesis of amino acids signaling pathway,and so on.The ovaries' transcriptome of Gaoyou ducks with high and low double-yolk performance were analyzed through RNA-Seq technology,the results provided the basis for mining and annotating new genes of Gaoyou ducks,and improved the data bases for the study of its regulation mechanism of double-yolk egg performance.

Key words: RNA-Seq; Gaoyou ducks; ovary; double-yolk egg; SNP

CLC Number:

S834

ZHANG Lei, ZHANG Haibo, ZHANG Jingqi, ZHANG Yang, CHANG Guobin, CHEN Guohong, ZHOU Lijun, WANG Jian. SNP Screening and Analysis of Ovaries Between Gaoyou Ducks with High and Low Yield Double-yolk Egg Based on RNA-Seq[J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(12): 3495-3503.

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SNP Screening and Analysis of Ovaries Between Gaoyou Ducks with High and Low Yield Double-yolk Egg Based on RNA-Seq

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