牛精子冷冻损伤及其改善方法

doi:10.16431/j.cnki.1671-7236.2021.06.023

Abstract

Abstract: The combination of cryopreservation of semen and artificial insemination technology plays an important role in the improvement of cattle breeds and the protection of excellent germplasm resources.Despite the motility of frozen bovine sperm is more than 60%,the damage and deficiency of frozen sperm are common,especially the fertilization ability is significantly lower than that of fresh sperm.This article introduced the main damage caused by freezing thawing process to bovine sperm,including the morphological integrity,vitality and genetic material changes of sperm.The main reasons of sperm damage caused by freezing were discussed,including the formation of ice crystals and oxidative stress in cells,and the possible mechanism.The common methods to improve the quality of frozen sperm,such as adding cryoprotectants,optimizing freezing procedures and adding antioxidants,were reviewed in detail.The main issue of sperm cryopreservation was discussed,and this article would provide a theoretical basis for enhanced cryopreservation of livestock semen.

Key words: bull; sperm; cryopreservation; cryo-damage; sperm quality

CLC Number:

S823

WANG Na, ZHANG Jie, HAI Chao, LI Xin, LI Guangpeng, ZHAO Yuefang. Cryo-damage of Bovine Sperm and Its Improvement[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(6): 2101-2112.

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Cryo-damage of Bovine Sperm and Its Improvement

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