虾青素对小鼠早期胚胎发育及相关基因表达的影响

doi:10.16431/j.cnki.1671-7236.2020.01.018

Abstract

Abstract: In present study,the effects of astaxanthin (AX) supplement in in vitro culture (IVC) medium on mouse embryo development and gene expression were determined.The female ICR strain mice (body weight 23-25 g) were super ovulated.The oocytes obtained from mouse oviduct were fertilized in vitro (IVF).The zygotes were divided randomly into 5 media suppled with different concentration of astaxanthin (0,5,10,25 and 50 μmol/L) for culture in vitro.Cultured for 24,96 h,the cleavage rate and blastocyst rate were analyzed.At 96 h,the cell number of blastocysts in each group was extracted.The total RNA of blastocysts in each group was abstracted,and relative expression amounts of TGF-β and Bcl-2 genes were determined by Real-time PCR.The results indicated that 10 μmol/L astaxanthin supplement could improve the in vitro development environment of mouse embryos and improve significantly embryo cleavage rate and blastocyst rate (P<0.05),increase cell number of blastocyst significantly (P<0.05),and increase expression amount of TGF-β gene extremely significantly (P<0.01).TGF-β and Bcl-2 genes were likely to be involved in the regulation of embryonic development.These results demonstrated that supplement of 10 μmol/L astaxanthin was beneficial to mouse embryo development in vitro,and could improve the developmental environment of mouse embryo.

Key words: astaxanthin; mouse; embryo; culture in vitro

CLC Number:

S814.6

ALAFAT·Amulati, ZHAO Xi, NURIBIYAMU·Maimaitituoheti, AERMAN·Haire, LIU GuoShi, ABULIZI·Wusiman. Effects of Astaxanthin on Mouse Implantation Embryos Development and Gene Expression[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(1): 148-155.

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Effects of Astaxanthin on Mouse Implantation Embryos Development and Gene Expression

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