牛源大肠杆菌对秀丽隐杆线虫的致病性研究

doi:10.16431/j.cnki.1671-7236.2020.03.030

Abstract

Abstract: In order to study the pathogenicity of Escherichia coli (E.coli)to Caenorhabditis elegans (C.elegans),13 strains of E.coli were isolated from cattle disease materials collected in Nanning,Guilin,Liuzhou and Guangxi in this study.Then,the E. coli were subjected to serological identification,mouse pathogenicity test and C.elegans pathogenicity test.The O serotype of E.coli were identified by slide agglutination and the 13 E.coli bacterial liquids were intraperitoneally injected into the mice at a concentration of 3.0×10⁹ CFU/mL and 0.2 mL/(10 g·BW).At the same time,these 13 strains of E. coli were infected with N2 wild type C.elegans.The results showed that the O serotypes of these E. coli were O127,O126 and O44,of which O126 was the dominant serotype (4/13).The pathogenicity results showed that 11 strains of E. coli could kill mice (40% to 100%),and 2 strains of E. coli were not lethal to mice (the mortality rates were 0). E.coli,which had strong pathogenicity to mice,also had a high lethal rate to nematodes,and the mortality rate were most significant on the 3rd to 6th day.The median lethal time was 3 to 4.5 d,and the longest survival time was 9 d.Two strains of E.coli which were not lethal to mice also had a low lethal rate to nematodes,the mortality of nematodes decreased slowly,the half lethal time was 5 to 6 d,and the longest survival time was 10 to 11 d.The results of intestinal bacterial count showed that the number of E.coli in the worm was linear with time,and E.coli constantly destroyed the nematode's immune system,which led to the death of nematodes.The results of this study indicated that the pathogenicity test of E.coli was consistent with the results of mouse pathogenicity test,indicating that the pathogenic model of E.coli-C.elegans was successfully established and laid the theoretical foundation for prevention and clinical use of bovine disease.

Key words: bovine; Escherichia coli; Caenorhabditis elegans; mouse; pathogenicity

CLC Number:

S852.61

BAI Huili, PENG Hao, LI Jun, LI Changting, TAO Li, CHEN Zhongwei, WU Cuilan, PAN Yan, ZENG Yun, GONG Yu. Study on the Pathogenicity of Escherichia coli from Cattle to Caenorhabditis elegans[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(3): 902-910.

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Study on the Pathogenicity of Escherichia coli from Cattle to Caenorhabditis elegans

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