版纳微型猪近交系白细胞介素6基因克隆、生物信息学及组织表达分析

doi:10.16431/j.cnki.1671-7236.2015.08.002

Abstract

Abstract: Interleukin-6 (IL-6) is an important immune regulatory factor, and has great development prospects in the application of adjuvants.Using the Banna minipig inbred line (BMI) as the experimental animal, we successfully cloned the coding region of pig IL-6 gene through polymerase chain reaction (PCR) method, and had deposited the sequence into NCBI database and assigned to the accession No.JQ839263.Then we further studied this gene by bioinformatics analysis method.The results showed that BMI IL-6 encoded 212 amino acids with a predicted molecular weight of 23.88 ku, the isoelectric point (pI) was 6.66 and a signal peptide in N-terminal.It was a nuclear protein with 89% probability.In addition, BMI IL-6 contained one conserved structure domain and one transmembrance structure, and its N-terminal and C-terminal were both hydrophobic.Comparing the sequence of the coding region of BMI IL-6 gene with the download 4 IL-6 gene sequences of pig, the result showed BMI was identical with GenBank accession No.NM_214399 and DQ832259, only had one base pair difference with GenBank accession No.AF309651 and AF518322, respectively.Multi-species amino acid sequence alignment and phylogenetic analysis demonstrated that BMI IL-6 showed the closest relationship with camel.Finally, we analyzed IL-6 gene mRNA expression in 12 important tissues of BMI through PCR method, the results showed that BMI IL-6 gene was highly expressed in lung, skin and muscle of BMI.These data laid a foundation for further insight into the expression and regulation of this gene of pig.

Key words: Banna minipig inbred line (BMI); IL-6; immune regulation; gene clone; tissue expression

CLC Number:

Q786

WANG Pei, CHEN Yuan-yuan, HUO Jin-long, HUO Hai-long, WANG Shu-yan, PAN Wei-rong, CHENG Wen-min, ZHA Xing-qin, ZENG Yang-zhi. Cloning,Bioinformatics and Tissue Expression Analysis of IL-6 Gene in Banna Minipig Inbred Line[J]. , 2015, 42(8): 1925-1934.

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Cloning,Bioinformatics and Tissue Expression Analysis of IL-6 Gene in Banna Minipig Inbred Line

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