穿王消炎粉对LPS诱导大鼠急性肺损伤的治疗作用

doi:10.16431/j.cnki.1671-7236.2022.04.039

Abstract

Abstract: 【Objective】 The experiment was conducted to study the therapeutic effect of Chuanwang Xiaoyan powder on lipopolysaccharide (LPS) induced acute lung injury in rats.【Methods】 60 SD male rats were randomly divided into blank group,levofloxacin-positive drug group,LPS model group,low-dose (1 g/kg BW),medium-dose (2 g/kg BW) and high-dose (4 g/kg BW) Chuanwang Xiaoyan powder groups.Each group contains 10 rats.A rat model of acute lung injury was made with 3 mg/kg LPS in the model group and the administration groups by the nasal drip method for 24 h.After successful modeling,the drug administration groups were given the corresponding doses of drugs by gavage,and the model and blank groups were given the same doses of saline by gavage.After 4 d of treatment,the rats were sacrificed,the serum was separated,and the lung tissues were fixed and cryopreserved.The wet/dry weight ratio of rat lung tissue was detected in each group.HE staining was used to observe the pathological changes of rat lung tissue.ELISA method was used to detect the content of IL-1β and IL-6 in serum,and the mRNA and protein expression levels of IL-1β and IL-6 in the lung tissue were respectively detected by Real-time quantitative PCR and Western blotting.【Results】 Compared with the blank group,rats in the LPS model group had thicker lung interstitial walls,infiltration of inflammatory cells in alveolar cavity,bleeding,and destruction of alveolar structure and other symptoms of pneumonia.The wet/dry weight ratio of lung tissue was increased extremely (P<0.01).The levels of IL-1β and IL-6 in serum,and the mRNA expression levels and protein expression levels of IL-1β and IL-6 in lung tissues were significantly increased (P<0.05).Compared with the LPS model group,the pulmonary interstitial thickening of the rats in the drug treatment groups was alleviated.The wet/dry weight ratio of lung tissue,the expression levels of inflammatory factors IL-1β,IL-6 mRNA and protein expressions in serum and lung tissue were significantly reduced (P<0.05).【Conclusions】 Chuanwang Xiaoyan powder could effectively inhibit LPS induced acute lung injury and inflammation.

Key words: Chuanwang Xiaoyan powder; LPS; rats; lung injury; IL-1β; IL-6

CLC Number:

S853.74

WANG Lu, HU Panpan, CHENG Jia, SUN Panpan, SUN Na, FAN Kuohai, YIN Wei, LI Hongquan, SUN Yaogui. Effect of Chuanwang Xiaoyan Powder on LPS Induced Acute Lung Injury in Rats[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1568-1576.

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Effect of Chuanwang Xiaoyan Powder on LPS Induced Acute Lung Injury in Rats

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