原儿茶酸对LPS诱导的小鼠子宫内膜炎的作用机制研究

doi:10.16431/j.cnki.1671-7236.2022.04.041

Abstract

Abstract: 【Objective】 This study was aimed to investigate the protective effect and mechanism of protocatechuic acid on lipopolysaccharide (LPS) induced endometritis in mice.【Method】 Sixty mice were randomly divided into control group,LPS group and LPS+ procatechuic acid (20,40,80 mg/kg) groups,with 12 mice in each group.The control group was injected with 50 mL normal saline via microsyringe,the LPS group was injected with 50 mL LPS (1 mg/kg),and the protocatechuic acid treatment groups were injected intraperitoneally with 20,40 and 80 mg/kg protocatechuic acid 1 h before the injection of 50 mL LPS.After 24 h of injection,all the mice were sacrificed by cervical dislocation.The uterus tissues were collected,and the pathological changes of the uterus tissues were detected by HE staining,myeloperoxidase (MPO) activity was detected by kit,and the contents of inflammatory factors tumor necrosis factor α(TNF-α),interleukin 1β(IL-1β) and IL-6 were detected by ELISA.The expression levels of p65 and NF-κB inhibitor protein (IκB) and phosphorylaed p65 and IκB protein (p-p65 and p-IκB) were detected by Western blotting.【Result】 Histopathological results showed that the endometrial epithelial structure of mice in control group was normal.The uterus tissues of mice in LPS group showed severe inflammatory cell infiltration and endometrial epithelial hyperemia and edema.Compared with LPS group,with the increase of protocatechuic acid concentration,the inflammatory cells in the uterus tissues of mice were significantly reduced,and the hyperemia and edema of endometrial epithelium were significantly improved.The activity of MPO in LPS group was extremely significantly higher than that in control group (P<0.01).Compared with LPS group,MPO activity in protocatechuic acid treatment group was extremely significantly decreased in a dose-dependent manner (P<0.01).ELISA results showed that compared with control group,the contents of TNF-α,IL-1β and IL-6 in endometrial tissues of mice in LPS group were extremely significantly increased (P<0.01).Compared with LPS group,the contents of inflammatory cytokines TNF-α,IL-1β and IL-6 in endometrial tissues of mice treated with protocatechuic acid were extremely significantly decreased (P<0.01) in a dose-dependent manner.Western blotting results showed that the expression of p-p65 and p-IκB in LPS group were extremely significantly increased compared with control group (P<0.01).Compared with LPS group,the expression of p-p65 and p-IκB in protocatechuic acid treatment group were extremely significantly decreased in a dose-dependent manner (P<0.01).【Conclusion】 Protocatechuic acid played a protective effect on lPS-induced endometritis in mice by alleviating pathological changes,decreasing MPO activity,inhibiting NF-κB signaling pathway and the production of inflammatory cytokines.

Key words: protocatechuic acid; lipopolysaccharide (LPS); endometritis; NF-κB signaling pathway

CLC Number:

S857.4

ZHANG Yuzhu, YU Chao, ZHENG Huihua, TANG Xinyue, HUANG Ronglei, XIE Guanghong. Mechanism Study of Protocatechuic Acid on LPS-induced Endometritis in Mice[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1585-1591.

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Mechanism Study of Protocatechuic Acid on LPS-induced Endometritis in Mice

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