短期灌喂民猪源贝莱斯芽孢杆菌M2对SD大鼠生长、免疫及肠道菌群的影响

doi:10.16431/j.cnki.1671-7236.2021.04.013

Abstract

Abstract: The effects of short-term feeding with Bacillus velezensis M2 isolated from Min pigs on the growth performance,blood biochemistry,immunity,and intestinal flora of SD rats were investigated for providing a reference for the application of Bacillus velezensis in livestock and poultry.A total of 24 healthy SD female rats of 21-day-old,similar weight and SPF grade,were randomly divided into two groups (six repeats per group,and two rats per repeat).Pre-feeding period lasted three days.During the trial period,the rats in control group were fed orally 0.2 mL normal saline per day for 7 days,while those in the experimental group were fed orally 0.2 mL bacterial solution per day for 7 days.At the age of 35- and 45-day-old,one rat in each repeat was randomly selected to be executed and sampled.The results showed that feeding with Bacillus velezensis M2 increased the final weight and daily gain,and decreased daily feed intake and feed-gain ratio (P>0.05).Short-term feeding with Bacillus velezensis M2 significantly reduced plasma triglyceride concentration,plasma IgA and TNF-α level of 35-day-old rats,and plasma IL-6 level of 45-day-old rats (P<0.05),and significantly increased plasma IgM level of 35-day-old rats (P<0.05).Short-term feeding with Bacillus velezensis M2 had no significant effect on intestinal flora of 35-day-old rats (P>0.05),but significantly reduced the proportion of Actinomycetes and Proteobacteria in 45-day-old rats (P<0.05).These results indicated that short-term feeding with the Bacillus velezensis M2 regulated the lipid metabolism,immunity and intestinal flora abundance in SD rats.

Key words: SD rats; Bacillus velezensis M2; Min pigs; intestine; microflora

CLC Number:

S816.3

SUN Changchao, GAO Xiang, LI Feng, LIANG Tong, NING Hangyi, WANG Zhongyu, SHAN Anshan, SHI Baoming. Effects of Short-term Administration of Bacillus velezensis M2 Isolated from Min Pigs on the Growth,Immunity and Intestinal Microflora of SD Rats[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(4): 1264-1274.

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Effects of Short-term Administration of Bacillus velezensis M2 Isolated from Min Pigs on the Growth,Immunity and Intestinal Microflora of SD Rats

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