香芹酚和百里香酚对断奶仔猪结肠微生物的影响研究

doi:10.16431/j.cnki.1671-7236.2020.12.012

Abstract

Abstract: This study was conducted to investigate the effects of carvacrol and thymol on the relative abundance of colonic microorganisms in weaned piglets and their metabolic pathways.A total of sixty four 28-day-old (8.5 kg±1.0 kg) weaned piglets were divided into 4 groups with 16 replicate of 1 piglet:Group A (control group,basal diet),group B (basal diet＋20 g/T carvacrol),group C (basal diet＋20 g/T thymol),and group D (basal diet＋20 g/T carvacrol-thymol blend (mass ratio 2:1)).The experiment lasted 30 d.The sequencing was taken based on the Illumina HiSeq sequencing platform,and using paired-end sequencing (Paired-End) method.Perform species annotation and abundance were analysized by clustering OTUs,and alpha diversity analysis、beta diversity analysis、significant species difference analysis and functional gene prediction analysis were further conducted.The results showed as follow:①The colonic microorganisms biodiversity of the experimental groups were significantly higher than that of control group (P<0.05).②The relative abundance of Firmicutes in the colon of group D was extremely significantly higher than that of control group (P<0.01),significantly higher than groups B and C (P<0.05),the relative abundance of Bacteroidetes was significantly higher than that of control group and group C (P<0.05),and the relative abundance of Epsilonbacteraeota,Spirochaetes and Proteobacteria were extremely significantly lower than that of control group (P<0.01).The relative abundance of Campylobacter in the colon of group D was extremely significantly lower than that of control group (P<0.01),that of Rikenellaceae_RC9_gut_group in group D was significantly higher than that of group B (P<0.05),that of Roseburia in group D was extremely significantly higher than that of group A (P<0.01),that of Helicobacter was extremely significantly lower than that of group A (P<0.01).③The lipid metabolism level of colonic microorganisms in group D was extremely significantly higher than that of control group (P<0.01),and colonic microbial carbohydrate metabolites,biosynthetic of other secondary metabolites and other amino acid metabolism level were significantly higher than that of control group (P<0.05),and energy metabolism level of colonic microorganisms was extremely significantly lower than that of control group (P<0.01).In conclusion,under the condition of this experiment,dietary supplementation with 20 g/t carvacrol-thymol improved the microbial community structure of colonic microorganisms in weaned piglets.The relative abundance of colonic beneficial bacteria was increased,the relative abundance of colonic harmful bacteria was decreased,and positively affected the metabolic function of colonic microorganisms.

Key words: carvacrol; thymol; weaned piglets; colonic microflora; microbial metabolic function

CLC Number:

S816.7

LIU Zhonghao, ZHENG Zi, CHEN Liang, LI Ning, LI Qianjun, MU Shuqin, YAN Jun. Effects of Carvacrol and Thymol on Colonic Microorganisms in Weaned Piglets[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(12): 3897-3908.

References

[1] BACKHED F,DING H,WANG T,et al.The gut microbiota as an environmental factor that regulates fat storage[J].Proceedings of the National Academy of Sciences of the United States of America,2004,101(44):15718-15723
[2] KOH A,DE VADDER F,KOVATCHEVA-DATCHARY P,et al.From dietary fiber to host physiology:Short-chain fatty acids as key bacterial metabolites[J].Cell,2016,165(6):1332-1345.
[3] LIANG X,ESTELLE J,KⅡLERICH P,et al.A reference gene catalogue of the pig gut microbiome[J].Nature Microbiology,2016,1(12):16161.
[4] 尹杰,韩慧,刘泽民,等.功能性氨基酸调控猪氧化应激的研究进展[J].中国科学:生命科学,2019,49(3):193-201. YIN J,HAN H,LIU Z M,et al.Regulation of functional amino acids on oxidative stress in pigs[J].Scientia Sinica(Vitae),2019,49(3):193-201.(in Chinese)
[5] 严佳豪,胡睿智,王楹,等.微生物、氧化应激与母乳在猪母子一体化营养调控中的作用[J].动物营养学报,2020,32(7):2981-2988. YAN J H,HU R Z,WANG Y,et al.Roles of microbe,oxidative stress and breast milk in regulation of maternal-child integrated nutrition in swine[J].Chinese Journal of Animal Nutrition,2020,32(7):2981-2988.(in Chinese)
[6] 于光辉,姜建阳,张华杰,等.仔猪断奶应激对肠道微生物菌群影响的研究[J].中国兽医杂志,2019,55(4):44-47. YU G H,JIANG J Y,ZHANG H J,et al.Effects of piglets weaning stress on intestine microflora[J].Chinese Journal of Veterinary Medicine,2019,55(4):44-47.(in Chinese)
[7] LI P H,NIU Q,WEI Q T,et al.Microbial shifts in the porcine distal gut in response to diets supple-mented with Enterococcus faecalis as alternatives to antibiotics[J].Scientific Reports,2017,7(1):41395.
[8] LALLES J P,BOSI P,SMIDT H,et al.Nutritional management of gut health in pigs around weaning[J].Proceedings of the Nutrition Society,2007,66(2):260-268.
[9] 李振翼,彭芳,贺喜,等.百里香酚调控动物肠道菌群的可能机制及其在畜禽生产中替代抗生素的应用[J].动物营养学报,2020,32(7):3072-3079. LI Z Y,PENG F,HE X,et al.Thymol:Possible mechanisms of controlling intestinal flora of animals and application in antibiotic substitution of livestock and poultry production[J].Chinese Journal of Animal Nutrition,2020,32(7):3072-3079.(in Chinese)
[10] KLEIN G,RUBEN C,UPMANN M.Antimicrobial activity of essential oil components against potential food spoilage microorganisms[J].Current Microbiology,2013,67:200-208.
[11] YAO L,HOU G,WANG L,et al.Protective effects of thymol on LPS-induced acute lung injury in mice[J].Microbial Pathogenesis,2018,116:8-12.
[12] 张有林,张润光,钟玉.百里香精油的化学成分、抑菌作用、抗氧化活性及毒理学特性[J].中国农业科学,2011,44(9):1888-1897. ZHANG Y L,ZHANG R G,ZHONG Y.Chemical component,antimicrobial effect,antioxidation activity and toxicological character of thyme essential oil[J].Scientia Agricultura Sinica,2011,44(9):1888-1897.(in Chinese)
[13] VAN NOTEN N,DEGROOTE J,VAN LIEFFERINGE E,et al.Effects of thymol and thymol α-D-glucopyrano-side on intestinal function and microbiota of weaned pigs[J].Animals,2020,10(2):329.
[14] MICHIELS J,MISSOTTEN J,VAN HOORICK A,et al.Effects of dose and formulation of carvacrol and thymol on bacteria and some functional traits of the gut in piglets after weaning[J].Archives of Animal Nutrition,2010,64(2):136-154.
[15] WEI H K,XUE H X,ZHOU Z X,et al.A carvacrol-thymol blend decreased intestinal oxidative stress and influenced selected microbes without changing the messenger RNA levels of tight junction proteins in jejunal mucosa of weaning piglets[J].Animal,2017,11(2):193-201.
[16] 李维,孙开济,孙玉丽,等.乳酸菌缓解肠道氧化应激研究进展[J].动物营养学报,2016,28(1):9-14. LI W,SUN K J,SUN Y L,et.al.Research progress in relieve effect of Lactobacillus in intestinal oxidative stress[J].Chinese Journal of Animal Nutrition,2016,28(1):9-14.(in Chinese)
[17] YANG J,QIAN K,WANG C.et al.Roles of probiotic lactobacilli inclusion in helping piglets establish healthy intestinal inter-environment for pathogen defense[J].Probiotics & Antimicro Proteins,2018,10:243-250.
[18] EDGAR R C.Uparse:Highly accurate OTU sequences from microbial amplicon reads[J].Nature Methods:Techniques for Life Scientists and Chemists,2013,10(10):996-998.
[19] BOKULICH N A,SUBRAMANIAN S,FAITH J J,et al.Quality-filtering vastly improves diversity estimates from Illumina amplicon sequencing[J].Nature Methods,2012,10(1):57-59.
[20] HILDEBRAND F,NGUYEN T L,BRINKMAN B,et al.Inflammation-associated enterotypes,host genotype,cage and inter-individual effects drive gut microbiota variation in common laboratory mice[J].Genome Biology,2013,14:(1):R4.
[21] MARCHESI J R,ADAMS D H,FAVA F,et al.The gut microbiota and host health:A new clinical frontier[J].Gut,2016,65(2):330.
[22] KIM H B,BOREWICZ K,WHITE B A,et al.Longitudinal investigation of the age-related bacterial diversity in the feces of commercial pigs[J].Veterinary Microbiology,2011,153(1-2):124-133.
[23] NIU Q,LI P,HAO S,et al.Characteristics of gut microbiota in sows and their relationship with apparent nutrient digestibility[J].International Journal of Molecular Sciences,2019,20(4):870-882.
[24] O'TOOLE P W,JEFFERY I B.Gut microbiota and aging[J].Science,2015,350(6265):1214-1215.
[25] TIAN L M,BRUGGEMAN G,VAN DEN BERG M,et al.Effects of pectin on fermentation characteristics,carbohydrate utilization,and microbial community composition in the gastrointestinal tract of weaning pigs[J].Molecular Nutrition & Food Research,2017,61(1):1600186.
[26] 张显东.香芹酚和百里香酚的作用机理及其在单胃动物中的应用研究进展[J].中国畜牧杂志,2017,53(11):25-30. ZHANG X D.The Mode of action and application of carvacrol and thymol in monogastric animals:A review[J].Chinese Journal of Animal Science,2017,53(11):25-30.(in Chinese)
[27] LESJAK M,SIMIN N,ORCIC D,et al.Binary and tertiary mixtures of satureja hortensis and origanum vulgare essential oils as potent antimicrobial agents against helicobacter pylori[J].Phytotherapy Research,2016,30(3):476-484.
[28] 谢强,林玉桓,苗淑萍,等.香芹酚对大肠杆菌和金黄色葡萄球菌细胞膜的影响[J].食品工业科技,2014,35(23):54-58. XIE Q,LIN Y H,MIAO S P,et al.Effects of carvacrol on Escherichia coli and Staphylococcus aureus membrane[J].Science and Technology of Food Industry,2014,35(23):54-58.(in Chinese)
[29] 戴雨芸,李超,袁中伟,等.香芹酚抑制金黄色葡萄球菌生物被膜的形成[J].微生物学通报,2020,47(3):813-820. DAI Y Y,LI C,YUAN Z W,et al.Inhibition of Staphylococcus aureus biofilm by carvacrol[J].Microbiology China,2020,47(3):813-820.(in Chinese)
[30] ZHOU W,WANG Z,MO H Z,et al.Thymol mediates bactericidal activity against Staphylococcus aureus by targeting an Aldo-Keto reductase and consequent depletion of NADPH[J].Journal of Agricultural and Food Chemistry,2019,67(30):8382-8392.
[31] 马玉芳,王冠淞,闵思明,等.牛至油对断奶仔猪生长性能、血清激素指标及小肠组织胰岛素样生长因子-Ⅰ含量的影响[J].动物营养学报,2018,30(6):2303-2309. MA Y F,WANG G S,MIN S M,et al.Effects of oregano essential oil on growth performance,serum hormone indexes and small intestine tissues insulin-like growth factor-Ⅰ content of weaned piglets[J] Chinese Journal of Animal Nutrition,2018,30(6):2303-2309.(in Chinese)
[32] WANG L L,GUO H H,HUANG S,et al.Comprehensive evaluation of SCFA production in the intestinal bacteria regulated by berberine using gas-chromatography combined with polymerase chain reaction[J].Journal of Chromatography B,2017,1057:70-80.
[33] DIAO H,JIAO A R,YU B,et al.Gastric infusion of short-chain fatty acids can improve intestinal barrier function in weaned piglets[J].Genes & Nutrition,2019,14(1):4-20.
[34] NICHOLSON J K,HOLMES E,KINROSS J,et al.Host-gut microbiota metabolic interactions[J].Science,2012,336(6086):1262-1267.
[35] LOUIS P,YOUNG P,HOLTROP G,et al.Diversity of human colonic butyrate-producing bacteria revealed by analysis of the butyryl-CoA:Acetate CoA-transferase gene[J].Environmental Microbiology,2010,12(2):304-314.
[36] RITZHAUPT A,ELLIS A,HOSIE K B,et al.The characterization of butyrate transport across pig and human colonic luminal membrane[J].The Journal of Physiology,1998,507(3):819-830.
[37] KASAHARA K,KRAUTKRAMER K A,ORG E,et al.Interactions between Roseburia intestinalis and diet modulate atherogenesis in a murine model[J].Nature Microbiology,2018,3(12):1461-1471.
[38] CANI P D,VAN HUL M,LEFORT C,et al.Microbial regulation of organismal energy homeostasis[J].Nature Metabolism,2019,1:34-46.
[39] SARAH J,STANISLAS M,JOSEF E,et al.The gut microbiota drives the impact of bile acids and fat source in diet on mouse metabolism[J].Microbiome,2018,6(1):134-152.
[40] DENTIN R,PEGORIER J P,BENHAMED F,et al.Hepatic glucokinase is required for the synergistic action of ChREBP and SREBP-1c on glycolytic and lipogenic gene expression[J].Journal of Biological Chemistry,2004,279(19):20314-20326.
[41] 梁源,于瑞丽,尹悦,等.肠道菌群及其代谢产物在糖脂代谢中的作用[J].生理科学进展,2019,50(5):321-325. LIANG Y,YU R L,YIN Y,et al.Effects of gut microbiota and its metabolite in glucose and lipid metabolism[J].Progress in Physiological Sciences,2019,50(5):321-325.(in Chinese)
[42] GROOTAERT C,WIELE T V D,ROOSBROECK I V,et al.Bacterial monocultures,propionate,butyrate and H₂O₂ modulate the expression,secretion and structure of the fasting-induced adipose factor in gut epithelial cell lines[J].Environmental Microbiology,2011,13(7):1778-1789.
[43] ARONSSON L,HUANG Y,PARINI P,et al.Decreased fat storage by Lactobacillus paracasei is associated with increased levels of angiopoietin-like 4 protein (ANGPTL4)[J].PLoS One,2010,5(9):e13087.
[44] 赵旭,徐群,侯彦茹,等.ANGPTL4在肠道微生物影响动物脂肪代谢中的作用[J].生物技术通报,2020,36(6):230-235. ZHAO X,XU Q,HOU Y R,et al.Effects of ANGPTL4 on intestinal microbiota affecting lipid metabolism of animals[J].Biotechnology Bulletin,2020,36(6):230-235.(in Chinese)
[45] EDWARD A B P,JONG-PYO C,MARILEN P B,et al.Assessment of fecal bacterial diversity among healthy piglets during the weaning transition[J].Journal of General & Applied Microbiology,2014,60(4):140-146.
[46] KOVATCHEVA-DATCHARY P,NILSSON A,AKRAMI R,et al.Dietary fiber-induced improvement in glucose metabolism is associated with increased abundance of Prevotella[J].Cell Metabolism,2015,22(6):971-982.
[47] PRECUP G,VODNAR D C.Gut Prevotella as a possible biomarker of diet and its eubiotic versus dysbiotic roles-A comprehensive literature review[J].British Journal of Nutrition,2019,122(2):1-24.
[48] GUIMARĀES A C,MEIRELES L M,LEMOS M F,et al.Antibacterial activity of terpenes and terpenoids present in essential oils[J].Molecules,2019,24(13):2471.

Effects of Carvacrol and Thymol on Colonic Microorganisms in Weaned Piglets

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