复合氨基酸对断奶仔猪肝脏能量状态、游离氨基酸含量及其相关基因表达的影响

doi:10.16431/j.cnki.1671-7236.2020.08.012

Abstract

Abstract: In order to further explore the application effects and mechanism of an amino acid blend (AAB),this study investigated the effects of dietary supplementation with AAB on liver energy state,free amino acids and relative expression of related genes in weaned piglets following our previous studies.Sixteen (21±3) days old weaned piglets with similar body weight were randomly assigned to control group and AAB group,with 8 replicates per group.The diet of the control group was basal diet supplemented with 0.99% alanine (isonitrogenous control),the diet of AAB group was basal diet supplemented with 1.00% AAB (glutamate:glutamine:glycine:arginine:N-acetylcysteine=5:2:2:1:0.5).On day 21 of the trial,the blood and liver samples were obtained from all piglets.The results indicated that:Compared to the control group,AAB group decreased plasma ALT and AST activity in piglets (P<0.05),increased the total protein content,RNA/DNA and total protein/DNA in the liver of piglets(P<0.05),reduced the MDA and H₂O₂ contents in the liver of piglets (P<0.05),increased the liver arginine and adenosine diphosphate (ADP) levels (P<0.05),increased mRNA levels of amino acid transporter b^0,+ AT and y⁺ LAT2 genes,glutathione S-transferase ω2 gene (GSTO2),interferon-γ gene (IFN-γ),lipoprotein lipase gene (LPL) (P<0.05),decreased mRNA levels of fatty acid synthase gene (FASN) (P<0.05).The above results showed that the addition of 1.00% AAB could improve the liver function,improve the antioxidant capacity,regulate the energy state and free amino acid metabolism of piglet liver,and might affect the fat metabolism and immune function of liver.

Key words: amino acid blend; weaned piglets; liver; energy state; free amino acids; gene

CLC Number:

S828.5

DONG Yi, WANG Qian, MENG Yuxuan, ZHU Zhekun, TIAN Junjie, WANG Lei, ZHAO Di, HOU Yongqing. Effects of Amino Acid Blend on Liver Energy State,Free Amino Acids and Relative Expression of Related Genes in Weaned Piglets[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(8): 2445-2453.

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Effects of Amino Acid Blend on Liver Energy State,Free Amino Acids and Relative Expression of Related Genes in Weaned Piglets

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