基于16S rRNA测序揭示杜洛克猪早期体重与肠道微生物的关系

doi:10.16431/j.cnki.1671-7236.2021.01.021

Abstract

Abstract: The purpose of this study was to explore the possible association of the intestinal microbiome with early-stage weight in Duroc pigs.80 days old weaned piglets with Lighter body weight (LBW,n=9) and heavier body weight (HBW,n=9) were selected from 91 individuals based on their body weights ranked.Fecal samples from piglets in the early grower phase were performed for microbial diversity,composition and predicted functionality by sequencing the V3-V4 region of 16S rRNA gene.The results showed that there was no significant differences in microbial Alpha diversity between HBW and LBW groups(P>0.05).The two dominant phyla detected in both groups were Firmicutes (65.62% and 66.57% in HBW and LBW groups) and Bacteroidetes (30.87% and 29.80% in HBW and LBW groups).Four dominant genera were Prevotella (23.82% and 20.84% in HBW and LBW groups),Lactobacillus (9.11% and 16.55% in HBW and LBW groups),norank_f_Ruminococcaceae (10.32% and 9.98% in HBW and LBW groups),and norank_f_Lachnospiraceae (6.33% and 4.53% in HBW and LBW groups).PCoA analysis showed that there were small differences in gut microbes among Duroc piglets,whereas,some weight-associated microbial compositional differences were revealed between HBW and LBW groups.At the genus level,the abundance of several genera,such as g_Kitasatospora,g_norank_o_Tremblayales,g_norank_f_Erysipelotrichaceae,g_unclassified_f_[Paraprevotellaceae] and g_Coprobacillus,were significantly higher in HBW group than in LBW group (P<0.05).The abundance of Corynebacterium in LBW group was significantly higher than that in HBW group (P<0.05).Moreover,the KEGG pathways analysis showed that the sesquiterpenoid biosynthesis,insulin signaling pathway,ascorbate and aldarate metabolism,and biosynthesis of ansamycins were significantly enriched in HBW group (P<0.05),however,the secretion system pathway was significantly enriched in LBW group (P<0.05).These results might provide insights into host-microbe interactions occurring in the early stage piglets and would be useful in contributing to healthy pig breeding.

Key words: gut microbes; weaned piglets; body weight; 16S rRNA sequencing

CLC Number:

S813.3

SI Jinglei, HUANG Ye, CHEN Kuirong, LYU Dongling, CHENG Feng, TIAN Weilong, LIU Xiaoxiao, LIANG Liang, LI Yueyue, FENG Lingli, GAO Jiuyu, MO Jiayuan, LIANG Jing, LAN Ganqiu. Evaluating of the Association Between Early-stage Weight and Gut Microbiota in Duroc Pigs Using 16S rRNA Sequencing[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(1): 190-199.

References

[1] LU D,TIEZZI F,SCHILLEBEECKX C,et al.Host contributes to longitudinal diversity of fecal microbiota in swine selected for lean growth[J].Microbiome,2018,6(1):4.
[2] XIAO L,ESTELLE J,KIILERICH P,et al.A reference gene catalogue of the pig gut microbiome[J].Nature Microbiology,2016,1(12):16161.
[3] RAMAYO C Y,MACH N,LEPAGE P,et al.Phylogenetic network analysis applied to pig gut microbiota identifies an ecosystem structure linked with growth traits[J].International Society for Microbial Ecology,2016,10(12):2973-2977.
[4] KATOULI M,FOO E,KVHN I,et al.Evaluation of the Phene Plate generalized microplate for metabolic fingerprinting and for measuring fermentative capacity of mixed bacterial populations[J].Journal of Applied Microbiology,1997,82(4):511-518.
[5] KONSTANTINOV S R,AWATI A A,WILLIAMS B A,et al.Post-natal development of the porcine microbiota composition and activities[J].Environmental Microbio-logy,2006,8(7):1191-1199.
[6] MANN E,SCHMITZ-ESSER S,ZEBELI Q,et al.Mucosa-associated bacterial microbiome of the gastrointestinal tract of weaned pigs and dynamics linked to dietary calcium-phosphorus[J].PLoS One,2014,9(1):e86950.
[7] SPREEUWENBERG M A,VERDONK J M,GASKINS H R,et al.Small intestine epithelial barrier function is compromised in pigs with low feed intake at weaning[J].Journal of Nutrition,2001,131(5):1520-1527.
[8] THOMPSON C L,WANG B,HOLMES A J.The immediate environment during postnatal development has long-term impact on gut community structure in pigs[J].International Society for Microbial Ecology,2008,2(7):739-748.
[9] MACH N,BERRI M,ESTELL J,et al.Early-life establishment of the swine gut microbiome and impact on host phenotypes[J].Environmental Microbiology Reports,2015,7(3):554-569.
[10] DOU S,GADONNA W P,ROME V,et al.Characterisation of early-life fecal microbiota in susceptible and healthy pigs to post-weaning diarrhoea[J].PLoS One,2017,12(1):e0169851.
[11] METZLER-ZEBELI B U,SCHMITZ-ESSER S,MANN E,et al.Adaptation of the cecal bacterial microbiome of growing pigs in response to resistant starch type 4[J].Applied and Environmental Microbiology,2015,81(24):8489-8499.
[12] PAJARILLO E A,CHAE J P,BALOLONG M P,et al.Characterization of the fecal microbial communities of Duroc pigs using 16S rRNA gene pyrosequencing[J].Asian-Australasian Journal of Animal Sciences,2015,28(4):584-591.
[13] YANG L,BIAN G,SU Y,et al.Comparison of faecal microbial community of Lantang,Bama,Erhualian,Meishan,Xiaomeishan,Duroc,Landrace,and Yorkshire sows[J].Asian-Australasian Journal of Animal Sciences,2014,27(6):898-906.
[14] HAN G G,LEE J Y,JIN G D,et al.Evaluating the association between body weight and the intestinal micro-biota of weaned piglets via 16S rRNA sequencing[J].Applied Microbiology and Biotechnology,2017,101(14):5903-5911.
[15] DING X,LAN W,LIU G,et al.Exploring possible associations of the intestine bacterial microbiome with the pre-weaned weight gaining performance of piglets in intensive pig production[J].Scientific Reports,2019,9(1):15534.
[16] CAPORASO J G,KUCZYNSKI J,STOMBAUGH J,et al.QIIME allows analysis of high-throughput community sequencing data[J].Nature Methods,2010,7(5):335-336.
[17] STANISLAWSKI M A,DABELEA D,WAGNER B D,et al.Pre-pregnancy weight,gestational weight gain,and the gut microbiota of mothers and their infants[J].Microbiome,2017,5(1):113.
[18] TURNBAUGH P J,BÄCKHED F,FULTON L,et al.Diet-induced obesity is linked to marked but reversible alterations in the mouse distal gut microbiome[J].Cell Host & Microbe,2008,3(4):213-223.
[19] TURNBAUGH P J,HAMADY M,YATSUNENKO T,et al.A core gut microbiome in obese and lean twins[J].Nature,2009,457(7228):480-484.
[20] WANG X,TSAI T,DENG F,et al.Longitudinal investigation of the swine gut microbiome from birth to market reveals stage and growth performance associated bacteria[J].Microbiome,2019,7(1):109.
[21] BERNARD K.The genus corynebacterium and other medically relevant coryneform-like bacteria[J].Journal of Clinical Microbiology,2012,50(10):3152-3158.
[22] RAHMANI N,KASHIWAGI N,LEE J,et al.Mannan endo-1,4-β-mannosidase from Kitasatospora sp.isolated in Indonesia and its potential for production of mannooligosaccharides from mannan polymers[J].Applied Microbiology and Biotechno-logy Express,2017,7(1):100.
[23] VAN IMMERSEEL F,BOYEN F,GANTOIS I,et al.Supplementation of coated butyric acid in the feed reduces colonization and shedding of Salmonella in poultry[J].Poultry Science,2005,84(12):1851-1856.
[24] OIKONOMOU G,TEIXEIRA A G,FODITSCH C,et al.Fecal microbial diversity in pre-weaned dairy calves as described by pyrosequencing of metagenomic 16S rDNA.Associations of Faecalibacterium species with health and growth[J].PLoS One,2013,8(4):e63157.
[25] MYER P R,WELLS J E,SMITH T P,et al.Microbial community profiles of the colon from steers differing in feed efficiency[J].Springer Plus,2015,4(1):454.
[26] OH J K,CHAE J P.Association between the body weight of growing pigs and the functional capacity of their gut microbiota[J].Journal of Animal Science,2020,91(1):e13418.
[27] ANDOH A,NISHIDA A,TAKAHASHI K,et al.Comparison of the gut microbial community between obese and lean peoples using 16S gene sequencing in a Japanese population[J].Journal of Clinical Biochemistry and Nutrition,2016,59(1):65-70.
[28] HAKKAK R,KOROURIAN S,FOLEY S L,et al.Assessment of gut microbiota populations in lean and obese Zucker rats[J].PLoS One,2017,12(7):e0181451.
[29] KAAKOUSH N O.Insights into the role of Erysipelotrichaceae in the human host[J].Frontiers in Cellular and Infection Microbiology,2015,5:84.
[30] BASKIN D G,FIGLEWICZ LATTEMANN D,SEELEY R J,et al.Insulin and leptin:Dual adiposity signals to the brain for the regulation of food intake and body weight[J].Brain Research,1999,848(1-2):114-123.
[31] HE M,FANG S,HUANG X,et al.Evaluating the contribution of gut microbiota to the variation of porcine fatness with the cecum and fecal samples[J].Front Microbiology,2016,7:2108.
[32] LAYDEN B T,ANGUEIRA A R,BRODSKY M,et al.Short chain fatty acids and their receptors:New metabolic targets[J].Translational Research:The Journal of Laboratory and Clinical Medicine,2013,161(3):131-140.
[33] CHADWICK M,TREWIN H,GAWTHROP F,et al.Sesquiterpenoids lactones:Benefits to plants and people[J].International Journal of Molecular Sciences,2013,14(6):12780-12805.
[34] GREEN E R,MECSAS J.Bacterial secretion systems:An overview[J].Microbiology Spectrum,2016,4(1):215-239.
[35] LEWIS JR C M,OBREGÓN-TITO A,TITO R Y,et al.The human microbiome project:Lessons from human genomics[J].Trends in Microbiology,2012,20(1):1-4.
[36] TAUCH A,BURKOVSKI A.Molecular armory or niche factors:Virulence determinants of Corynebacterium species[J].FEMS Microbiology Letters,2015,362(23):fnv185.

Evaluating of the Association Between Early-stage Weight and Gut Microbiota in Duroc Pigs Using 16S rRNA Sequencing

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	WANG Yuan, WANG Wenwen, AN Xiaoping, QI Jingwei. Effects of Dietary Fermented Soybean Meal Replacement on Growth Performance, Digestive Enzyme,and Microbita Composition in Weaned Piglets [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 115-124.
[2]	CHEN Xueying, WANG Huisheng, WU Jie, DENG Bo, DIAO Xinping. Effects of Dietary Bacillus licheniformis and Bacillus amyloliquefaciens on Growth Performance and Blood Indicators of Weaned Piglets [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2547-2556.
[3]	ZHOU Di, AO Ye, JIANG Guirong, ZHAO Zhonghai, LI Jun, YANG Rong, GONG Fei, CHEN Changxue, SHEN Yin, LI Hui. Polymorphism of MEF2C Gene Exon and Its Association Analysis with Weight and Body Size Traits in Qiandongnan Xiaoxiang Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2613-2621.
[4]	WANG Chaopu, MA Xianyong, TIAN Zhimei, YU Miao, LIU Zhichang, DENG Dun, RONG Ting, TI Junling, SONG Min, ZHANG Huihua. Effects of Compound Chinese Medicine Fermentation Powder on Growth Performance,Nutrient Apparent Digestibility,Antioxidant Function and Immune Function of Weaned Piglets [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1322-1331.
[5]	YANG Zhiwei, LI Zhixing, HUANG Tao, HAO Kexing, WANG Jing, HU Guangdong. Effects of Early Weaning and Transfer on Behavior, Growth Performance and Blood Indexes of Piglets [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 904-912.
[6]	JIANG Changjin, CHEN Yun, PAN Pengcheng, CHEN Baojian, GUAN Zhihui, LU Huilin, XIE Bingkun, QIN Zhaoxian. Fecal Microflora Structure and Its Correlation with Growth Rate in Finishing Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 924-931.
[7]	BAI Peitian, KONG Jiamei, PEI Ting, CHENG Zhixue, REN Yuhong. Effects of Compound Microecological Preparation on Growth Performance, Immune Function and Caecal Flora Structure of Weaned Piglets [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 942-952.
[8]	ZHANG Yuying, LIU Shujie, FENG Yuzhe, ZHANG Xiaowei, CUI Zhanhong. Bacterial Flora Composition of Normal and Diarrhea Yak Calves Based on 16S rRNA Sequencing [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(9): 3293-3302.
[9]	XU Pan, ZHONG De, MA Zheng, LIU Linyu, WANG Yihui, ZHAO Xuting. Identification of Candidate Genes for Body Weight in Sujiang Pigs Based on Genome-wide Differential Methylation Analysis [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 2889-2900.
[10]	XU Lei, ZHANG Menghua, WANG Dan, ZHANG Xiaoxue, FAN Shoumin, LIU Jianming, LIU Jiangwei, YAN Chengxu, HUANG Xixia, WANG Yachun. Estimation of Genetic Parameters of Body Size and Body Weight in Xinjiang Brown Cattle at Different Growth Stages [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(11): 4105-4114.
[11]	DONG Yi, WANG Qian, MENG Yuxuan, ZHU Zhekun, TIAN Junjie, WANG Lei, ZHAO Di, HOU Yongqing. Effects of Amino Acid Blend on Liver Energy State,Free Amino Acids and Relative Expression of Related Genes in Weaned Piglets [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(8): 2445-2453.
[12]	LIU Yadan, WANG Haizhen, LIU Juan, WANG Shu, ZUO Zhou, CAI Chunbo, GUO Xiaohong, CAO Guoqing, LI Bugao, GAO Pengfei. Research Advance on Intestinal Microflora and Its Regulatory Factors of Piglets [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(7): 2005-2012.
[13]	LIU Xiangping, ZHU Jing, BAI Hao, KONG Linglin, GU Huabing, DOU Xinhong. Correlation Between Growth Traits and Immune Indexes of Xueshan Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(5): 1481-1488.
[14]	LIU Zhonghao, ZHENG Zi, CHEN Liang, LI Ning, LI Qianjun, MU Shuqin, YAN Jun. Effects of Carvacrol and Thymol on Colonic Microorganisms in Weaned Piglets [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(12): 3897-3908.
[15]	LI Hongmin, DONG Li, HU Zhenzhen, MAO Junzhou, ZHANG Yongsheng, LIU Jun, YU Lihuai. Effects of Attapulgite Nano Zinc Oxide on Antioxidant Function and Immune Function in Spleen of Weaned Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(11): 3484-3492.