恩诺沙星与磺胺二甲嘧啶联合毒性探究

doi:10.16431/j.cnki.1671-7236.2021.10.040

Abstract

Abstract: The aim of this study was to evaluate the combined toxicity of enrofloxacin and sulfamethazine. SD rats were selected and divided into 6 groups:High dose group (500 mg/(kg·BW)), middle dose group (250 mg/(kg·BW)), low dose group (50 mg/(kg·BW)), enrofloxacin group (250 mg/(kg·BW)), sulfamethazine group (250 mg/(kg·BW)) and control group 0.5% CMC, each group contained 12 rats. The corresponding drugs were prepared for intragastric administration. 1 d after the last administration, rats were weighed, heart blood was collected and liver tissues were dissected. Then the weight gain rate, blood routine indicators, serum biochemical indicators and liver pathological changes of the rats were analyzed, and the combined effect of the two drugs was evaluated by SPSS 22.0 software. The results showed that, in female rats, a significant decline of the weight gain rate was found at high and middle dose groups (P<0.05), but there was no significant change in male rats (P>0.05). Leukocyte populations in male rats had a significant rise (P<0.05), neutrophil and lymphocyte ratios were significantly increased and decreased at high dose group, respectively (P<0.05). The content of peripheral blood AST of male rats was significantly increased at high and middle dose groups (P<0.05), and liver pathologic reports revealed different degrees of inflammatory cell infiltration with different degrees of injury at high and middle dose groups. This study showed that mixing enrofloxacin and sulfamethazine might increase toxicity, it had a certain effect on the rat immune system, and could cause liver damage, and the higher the dose, the greater the effect. This work provided data support for the joint toxicity mechanism of enrofloxacin and sulfamethazine, and provided references for their clinical application. In addition, it suggested that new food safety assessment should consider the effects caused by co-exposure to multiple drugs.

Key words: enrofloxacin; sulfamethazine; SD rats; combined toxicity

CLC Number:

S859.79⁺6

ZHAO Junjie, CHEN Kexin, LUAN Yehui, CHENG Linli. Evaluation of Combined Toxicity of Enrofloxacin and Sulfamethazine[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(10): 3872-3879.

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Evaluation of Combined Toxicity of Enrofloxacin and Sulfamethazine

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